(1H-azol-1-ylmethyl)substituted quinoxaline derivatives

ABSTRACT

(1H-azol-1-ylmethyl)substituted quinoxaline derivatives, compositions containing the same, and methods of treating mammals suffering from disorders which are characterized by an increased proliferation and/or abnormal differentiation of epithelial tissues.

BACKGROUND OF THE INVENTION

In the European Patent Application No. 260,744, published Mar. 3, 1988,which corresponds to U.S. Pat. No. 4,859,684, there are described(1H-azol-1-ylmethyl) substituted benzimidazole derivatives whichcompounds are useful as androgenic hormone biosynthesis inhibitors. Thecompounds of the present invention differ from the cited art compoundsby the fact that they contain a quinoxaline moiety in place of abenzimidazole moiety and by their favourable pharmaceutical properties.In particular the compounds of the invention suppress the plasmaelimination of retinoic acids. Further it was found that some compoundsof the invention inhibit the formation of androgens from progestinesand/or inhibit the action of the enzyme complex aromatase whichcatalyses the formation of estrogens from androgenic steroids inmammals.

DESCRIPTION OF THE INVENTION

The present invention is concerned with novel compounds of formula##STR1## the pharmaceutically acceptable acid addition salts thereof andthe stereochemically isomeric forms thereof, wherein

--X¹ ═X² -- is a bivalent radical having the formula

    --CH═CH--                                              (x),

    --CH═N--                                               (y),

or

    --N═CH--                                               (z);

R is hydrogen or C₁₋₆ alkyl;

Y is hydrogen; C₁₋₁₀ alkyl; C₃₋₇ cycloalkyl; Ar¹ ; Ar² -C₁₋₆ alkyl; C₂₋₆alkenyl or

C₂₋₆ alkynyl;

Z is a radical of formula ##STR2## wherein R¹ is hydrogen, halo, C₁₋₆alkyl, C₁₋₆ alkyloxy, amino, mono- or di(C₁₋₆ alkyl)amino, Ar² orimidazolyl;

R² is hydrogen, C₁₋₆ alkyl or Ar¹ ;

R³ and R⁷ each independently are hydrogen, C₁₋₆ alkyl, Ar² -C₁₋₆ alkyl,amino or mono (C₁₋₆ alkyl)amino;

R⁴ and R⁸ each independently are hydrogen, C₁₋₆ alkyl, Ar¹, C₁₋₆alkylcarbonyl, Ar² -carbonyl, C₁₋₆ alkyloxycarbonyl, carboxyl, C₁₋₆alkyloxycarbonylC₁₋₄ alkyl, aminocarbonyl or cyano;

R⁵, R⁶, R⁹, R¹⁰ and R¹¹ each independently are hydrogen, C₁₋₆ alkyl orAr² -C₁₋₆ alkyl;

n is 0 or 1; and

Ar¹ is phenyl, substituted phenyl, naphthalenyl, pyridinyl, imidazolyl,triazolyl, thienyl, furanyl or thiazolyl and Ar² is phenyl orsubstituted phenyl; said substituted phenyl in Ar¹ or Ar² being phenylsubstituted with 1, 2 to 3 substituents each independently selected fromhalo, hydroxy, trifluoromethyl, C₁₋₆ alkyl, C₁₋₆ alkyloxy, cyano, amino,mono- and di(C₁₋₆ alkyl)amino, nitro, carboxyl, formyl and C₁₋₆alkyloxycarbonyl.

As used in the foregoing definitions the term halo is generic to fluoro,chloro, bromo and iodo; the term "C₁₋₆ alkyl" is meant to includestraight chained and branched saturated hydrocarbon radicals having from1 to 6 carbon atoms such as, for example, methyl, ethyl, 1-methylethyl,1,1-dimethylethyl, propyl, 2-methylpropyl, butyl, pentyl, hexyl and thelike; "C₁₋₁₀ alkyl" is meant to include the higher homologs of "C₁₋₆alkyl" containing 1-10 carbon atoms; the term "C₃₋₇ cycloalkyl" isgeneric to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl andcycloheptyl; "C₂₋₆ alkenyl" defines straight chained and branchedhydrocarbon radicals containing one double bond having from 2 to 6carbon atoms such as, for example, ethenyl, 2-propenyl, 3-butenyl,2-butenyl, 2-pentenyl, 3-pentenyl, 3-methyl-2-butenyl and the like;"C₂₋₆ alkynyl" defines straight chained and branched hydrocarbonradicals containing one triple bond and having from 2 to 6 carbon atomssuch as, for example, 2-propynyl, 2-butynyl, 3-butynyl, 2-pentynyl,3-pentynyl, 4-pentynyl and the like.

It is to be understood that the ##STR3## moiety hereinafter referred asthe 1H-azol-1-ylmethyl moiety may be substituted on either the 5, 6, 7or 8 position of the bicyclic ring system, the 6 or 7 position beingpreferred.

Further it should be noted that the compounds of formula (I) wherein Zis a radical of formula (a-1) are denoted as compounds of formula(I-a-1); compounds of formula (I) wherein Z is a radical of formula(a-2) are denoted as compounds of formula (I-a-2); compounds of formula(I) wherein Z is a radical of formula (a-3) are denoted as compounds offormula (I-a-3); compounds of formula (I) wherein Z is a radical offormula (a-4) are denoted as compounds of formula (I-a-4); and compoundsof formula (I) wherein Z is a radical of formula (a-5) are denoted ascompounds of formula (I-a-5).

The acid addition salts as mentioned hereinabove are meant to comprisethe therapeutically active non-toxic acid addition salt forms which thecompounds of formula (I) are able to form. The latter can convenientlybe obtained by treating the base form with appropriate acids such as,for example, inorganic acids, such as hydrohalic acid, e.g.hydrochloric, hydrobromic and the like, and sulfuric acid, nitric acid,phosphoric acid and the like; or organic acids such as, for example,acetic, hydroxyacetic, propanoic, 2-hydroxypropanoic, 2-oxopropanoic,ethanedioic, propanedioic, butanedioic, (Z)-2-butenedioic,(E)-2-butenedioic, 2-hydroxybutanedioic, 2,3-dihydroxybutanedioic,2-hydroxy-1,2,3-propanetricarboxylic, methanesulfonic, ethanesulfonic,benzenesulfonic, 4-methylbenzenesulfonic, cyclohexanesulfamic,2-hydroxybenzoic, 4-amino-2-hydroxybenzoic and the like acids.Conversely the salt form can be converted by treatment with alkali intothe free base form. The term acid addition salt also comprises thehydrates and solvent addition forms which the compounds of formula (I)are able to form. Examples of such forms are e.g. hydrates, alcoholatesand the like.

From formula (I) it is evident that the compounds of this invention mayhave several asymmetric carbon atoms in their structure. Pure isomericforms of the compounds of formula (I) can be separated from the mixtureby conventional separation methods. Preferably, if a specificstereoisomer is desired, said compound will be synthesized bystereoselective methods of preparation. These methods willadvantageously employ enantiomerically pure starting materials.

Further it is evident that the compounds of formula (I) may also containin their structure a tautomeric system and consequently these compoundscan be present in each of their tautomeric forms.

Particular compounds of the present invention are those compounds offormula (I) wherein R is hydrogen or C₁₋₄ alkyl; and/or Y is hydrogen,C₁₋₆ alkyl, C₃₋₇ cycloalkyl, phenyl, substituted phenyl, pyridinyl,imidazolyl or thienyl; and/or Z is a radical of formula (a-1), (a-2),(a-3), (a-4) or (a-5) wherein R¹ is hydrogen, C₁₋₄ alkyl, halo, C₁₋₄alkyloxy, amino, mono- or di(C₁₋₄ alkyl)amino, phenyl, substitutedphenyl or imidazolyl, R² is hydrogen, C₁₋₄ alkyl, phenyl or substitutedphenyl, R³ is hydrogen, C₁₋₄ alkyl, amino, C₁₋₄ alkylamino or C₁₋₄ alkylsubstituted with phenyl or substituted phenyl; R⁴ is hydrogen, C₁₋₄alkyl, C₁₋₄ alkyloxycarbonylC₁₋₄ alkyl, phenyl, substituted phenyl,carboxyl, C₁₋₄ alkyloxycarbonyl, carboxylyl, phenylcarbonyl, substitutedphenylcarbonyl, naphthalenyl, thienyl, furanyl, pyridinyl or imidazolyl;R⁵ and R⁶ each independently are hydrogen or C₁₋₄ alkyl; R⁷ is hydrogen,C₁₋₄ alkyl, amino or C₁₋₄ alkyl substituted with phenyl or substitutedphenyl; R⁸ is hydrogen, C₁₋₄ alkyl, phenyl, substituted phenyl, C₃₋₇cycloalkyl, naphthalenyl, thienyl, pyridinyl or imidazolyl; R⁹ ishydrogen or C₁₋₄ alkyl and R¹⁰ and R¹¹ are hydrogen.

More particular compounds are those particular compounds wherein --X¹═X² -- is a radical having the formula (x) or (y); and/or R is hydrogen;and/or Y is hydrogen, C₁₋₄ alkyl, cyclopropyl, cyclopentyl, cyclohexyl,imidazolyl, thienyl, pyridinyl or phenyl optionally substituted with oneor two substituents selected from halo, C₁₋₄ alkyl, C₁₋₄ alkyloxy andtrifluoromethyl.

Among the compounds of the aforementioned subgroups special emphasis isput on compounds of formula (I) wherein Z is a radical of formula (a-1)wherein R¹ is hydrogen, C₁₋₄ alkyl, C₁₋₄ alkyloxy, halo, amino, di(C₁₋₄alkyl)amino, phenyl or imidazolyl, R² is hydrogen, C₁₋₄ alkyl or phenyland Y is hydrogen, C₁₋₄ alkyl, thienyl, imidazolyl or phenyl optionallysubstituted with one or two substituents selected from halo, C₁₋₄ alkyl,C₁₋₄ alkyloxy or trifluoromethyl; and compounds of formula (I) wherein Zis a radical of formula (a-2) wherein R³ is hydrogen, C₁₋₄ alkyl, aminoor C₁₋₄ alkyl substituted with phenyl and R⁴ is hydrogen, C₁₋₄ alkyl,carboxyl, C₁₋₄ alkyloxycarbonyl, naphthalenyl, thienyl, pyridinyl,imidazolyl, phenyl or phenyl substituted with 1, 2 or 3 substituentseach independently selected from C₁₋₄ alkyl, C₁₋₄ alkyloxy, halo,hydroxy and trifluoromethyl and Y is hydrogen, C₁₋₄ alkyl, cyclopropyl,cyclopentyl, cyclohexyl, imidazolyl, thienyl, pyridinyl or phenyloptionally substituted with one or two substituents selected from halo,C₁₋₄ alkyl, C₁₋₄ alkyloxy and trifluoromethyl.

Preferred compounds of formula (I) wherein Z is a radical of formula(a-1) are those compounds wherein --X¹ ═X² -- is a radical having theformula (x) or (y); R is hydrogen; R¹ and R² are both hydrogen and Y isphenyl, halophenyl or thienyl.

Most preferred compounds of formula (I) wherein Z is a radical offormula (a-1) are selected from6-[(1H-imidazol-1-yl)phenylmethyl]quinoxaline and 6-[(4-fluorophenyl)(1H-imidazol-1-yl)methyl]quinoxaline, the pharmaceutically acceptableacid addition salts and possible stereochemically isomeric formsthereof.

Preferred compounds of formula (I) wherein Z is a radical of formula(a-2) are those compounds wherein --X¹ ═X² -- is a radical of formula(x) or (y); R is hydrogen; Y is hydrogen, C₁₋₄ alkyl, cyclopropyl,cyclopentyl or cyclohexyl; R³ is hydrogen; R⁴ is hydrogen, C₁₋₄ alkyl,naphthalenyl, thienyl, pyridinyl, imidazolyl, phenyl or phenylsubstituted with 1 or 2 substituents each independently selected frommethyl, halo, hydroxy and methoxy; and n is 0.

Other preferred compounds of formula (I) wherein Z is a radical offormula (a-2) are those compounds wherein --X¹ ═X² -- is a radical offormula (x) or (y); Y is phenyl or halophenyl; R³ is hydrogen; R⁴ ishydrogen or C₁₋₄ alkyl and n is 0.

Most preferred compounds of formula (I) wherein Z is a radical offormula (a-2) are selected from6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-phenyl-2(1H)-quinoxalinone,6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-propyl-2(1H)-quinoxalinone,3-(3-fluorophenyl)-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone, thepharmaceutically acceptable acid addition salts and possiblestereochemically isomeric forms thereof.

The compounds of formula (I) can be prepared by N-alkylating an azole offormula (II) or an alkali metal salt thereof with a quinoxalinederivative of formula (III). ##STR4## In formula (III) W represents anappropriate reactive leaving group such as, for example, halo, e.g.,fluoro, chloro, bromo, iodo or a sulfonyloxy group, e.g.4-methylbenzenesulfonyloxy, benzenesulfonyloxy,2-naphthalenesulfonyloxy, methanesulfonyloxy,trifluoromethanesulfonyloxy and the like reactive leaving groups.

The above described N-alkylation is conveniently carried out by stirringthe reactants in the presence of a suitable solvent such as, forexample, an aromatic hydrocarbon, e.g. benzene, methylbenzene,dimethylbenzene, and the like; an ester, e.g. ethyl acetate,γ-butyrolacetone and the like; a ketone, e.g. 2-propanone,4-methyl-2-pentanone and the like; an ether, e.g. 1,4-dioxane,1,1'-oxybisethane, tetrahydrofuran and the like; a polar aproticsolvent, e.g. N,N-dimethylformamide, N,N-dimethylacetamide, dimethylsulfoxide, 1-methyl-2-pyrrolidinone, acetonitrile, hexamethylphosphortriamide,1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone,1,3-dimethyl-2-imidazolidinone,benzonitrile and the like; or a mixture of such solvents. Somewhatelevated temperatures may be appropriate to enhance the rate of thereaction and in some cases the reaction may even be carried out at thereflux temperature of the reaction mixture. The addition of anappropriate base such as, for example, an alkali or an earth alkalinemetal carbonate, hydrogen carbonate, hydroxide, amide or hydride, e.g.sodium hydroxide, potassium hydroxide, potassium carbonate, sodiumhydride and the like or an organic base, such as, for example,N,N-dimethyl-4-pyridinamine, pyridine, N,N-diethylethanamine orN-(1-methylethyl)-2-propanamine may be employed to pick up the acidwhich is liberated during the course of the reaction. In some instancesit may be advantageous to use an excess of the azole (II) or to convertthe azole first into a suitable salt form thereof such as, for example,an alkali or earth alkaline metal salt, by reacting (II) with anappropriate base as defined hereinabove and subsequently using said saltform in the reaction with the alkylating reagent of formula (III).Additionally, it may be advantageous to conduct said N-alkylationreaction under an inert atmosphere such as, for example, oxygen-freeargon or nitrogen gas. Said alkylation may also be carried out byapplying art-known conditions of phase transfer catalysis reactions.

Compounds of formula (I) wherein --X¹ ═X² -- is a bivalent radical offormula (x), said compounds being represented by formula (I-x), may alsobe prepared by reacting a quinoxaline of formula (III) with a1-protected imidazole of formula (II-x) following the N-alkylationprocedures described hereinabove for the preparation of compounds offormula (I) starting from (II) and (III). ##STR5##

In (II-x) P¹ represents a protective group such as, for example, C₁₋₆alkylcarbonyl, C₁₋₆ alkyloxycarbonyl, arylcarbonyl or a tri(C₁₋₆alkyl)silyl group. In some instances the reaction of (II-x) with (III)first yields a 1-protected imidazolium salt of formula (IV) which may insitu, or if desired, after isolating and further purifying it, bedeprotected by stirring it in an aqueous basic solution or acidicsolution. ##STR6##

In (IV) W⁻ is an anion arising from an acid such as, for example,hydrochloric acid, hydrobromic acid, methanesulfonic acid,4-methylbenzenesulfonic acid and the like acids.

Compounds of formula (I) wherein --X¹ ═X² -- is a bivalent radical offormula (y), said compounds being represented by formula (I-y), can alsobe prepared by endo-N-alkylation of a triazolamine of formula (II-y)with a quinoxaline of formula (III) and subsequent deamination of thethus prepared triazolium salt, wherein W⁻ is an anion as definedhereinabove. ##STR7##

The endo-N-alkylation reaction of (II-y) with (III) is carried outaccording to similar procedures as described hereinabove for thepreparation of a compound of formula (I) starting from (III) and (II).Said deamination reaction is conveniently conducted by reaction with anacidic nitrite solution in the presence of an appropriate reductant, orby reaction with an alkylnitrite such as, for example,1,1-dimethylethylnitrite or isoamylnitrite and the like. Preferably,said deamination reaction is conducted with an aqueous solution ofnitrous acid or of a nitrite salt in a suitable acid in the presence ofa reducing agent such as, for example, hypophosphorous acid, formicacid, at a lower temperature.

The compounds of formula (I) may also be prepared by reacting anintermediate of formula (V) with a reagent of formula (VI) such as, forexample, a 1,1'-carbonylbis[1H-imidazole], 1,1'-carbonylbistriazole.##STR8## In (VI) X represents C or S.

Said reaction may conveniently be conducted in a suitable solvent suchas, for example, an ether, e.g. 1,4-dioxane, tetrahydrofuran; ahalogenated hydrocarbon, e.g. di- or trichloromethane; a hydrocarbon,e.g. benzene, methylbenzene, dimethylbenzene; N,N-dimethylformamide,N,N-dimethylacetamide, or a mixture of such solvents. In order toenhance the reaction rate, it may be advantageous to heat the reactionmixture.

The compounds of formula (I) may also be prepared by reacting a ketoneor aldehyde of formula (VII) with an azole (II) in the presence offormic acid or formamides as reducing agents. ##STR9##

Said reductive alkylation can conveniently be conducted by stirring andheating the reagents in formic acid or formamides optionally in thepresence of an acid catalyst. An appropriate acid catalyst for using inthis reaction is for example a mineral acid such as, hydrochloric acid,sulfuric acid or a sulfonic acid such as, methanesulfonic acid,benzenesulfonic acid, 4-methylbenzenesulfonic acid and the like. It maybe appropriate to remove the water which is formed during the reactionby azeotropical distillation, distillation, complexation and the likemethods.

In all of the foregoing and following preparations, the reactionproducts may be isolated from the reaction mixture and, if necessary,further purified according to methodologies generally known in the artsuch as, for example, extraction, distillation, crystallization,trituration and chromatography.

The compounds of formula (I) can alternatively be prepared under similarconditions as are described in the literature by condensing anappropriate ortho-disubstituted benzene with a two-carbon synthon.

The compounds of formula (I) wherein Z is a radical (a-1) and R¹ ishydrogen, C₁₋₆ alkyl or Ar², said compound being represented by formula(I-a-1-a), may be obtained by condensing an appropriateortho-benzenediamine of formula (VIII-a) with a 1,2-diketone of formula(IX). ##STR10##

In (IX) and (I-a-1-a) R^(1-a) represents hydrogen, C₁₋₆ alkyl or Ar².

The condensation of the (1H-azol-1-ylmethyl) substituted ortho-diamineof formula (VIII-a) and the 1,2-diketone of formula (IX) can be carriedout by mixing the reactants in a suitable solvent such as, for example,an alkanol, e.g. methanol, ethanol, propanol and the like; an ether,e.g. tetrahydrofuran, 1,4-dioxane, 1,1'-oxybisbutane and the like; ahalogenated hydrocarbon, e.g. trichloromethane, dichloromethane and thelike; a dipolar aprotic solvent, e.g., N,N-dimethylformamide,N,N-dimethylacetamide, dimethylsulfoxide; an aromatic hydrocarbon, e.g.benzene, methylbenzene, dimethylbenzene and the like or mixtures of suchsolvents optionally in the presence of a carboxylic acid, e.g. aceticacid and the like, a mineral acid such as, for example hydrochloricacid, sulfuric acid, or a sulfonic acid such as, for example,methanesulfonic acid, benzenesulfonic acid, 4-methylbenzenesulfonic acidand the like. Somewhat elevated temperatures may be appropriate toenhance the rate of the reaction and in some cases the reaction may evenbe carried out at the reflux temperature of the reaction mixture. Thewater which is liberated during the condensation may be removed from themixture by azeotropical distillation, distillation and the like methods.As suitable 1,2-diketones of formula (IX) there may be named forexample, ethanedial, diphenylethanedione, 2,3-butanedial and the liketwo carbon synthons.

The compounds of formula (I) wherein Z is a radical of formula (a-2) andn is 0, said compounds being represented by (I-a-2-a), may be obtainedby condensing an appropriate ortho-benzenediamine of formula (VIII-b)with an appropriate α-keto acid of formula (X) or a functionalderivative thereof such as, for example, an ester, a halide and thelike. ##STR11##

The condensation of the (1H-azol-1-ylmethyl) substituted ortho-diamineof formula (VIII-b) and the α-keto acid or ester of formula (X) can becarried out by mixing the reactants in a suitable solvent such as, forexample, water, an alkanol, e.g. methanol, ethanol, propanol and thelike; an ether, e.g. tetrahydrofuran, 1,4-dioxane, 1,1'-oxybisbutane andthe like; a halogenated hydrocarbon, e.g. trichloromethane,dichloromethane and the like; a dipolar aprotic solvent, e.g.N,N-dimethylformamide, N,N-dimethylacetamide, dimethylsulfoxide, and thelike; an aromatic hydrocarbon, e.g. benzene, methylbenzene,dimethylbenzene and the like; and mixtures of such solvents optionallyin the presence of a carboxylic acid, e.g. acetic acid and the like, amineral acid such as, for example hydrochloric acid, sulfuric acid, or asulfonic acid such as, for example, methanesulfonic acid,benzenesulfonic acid, 4-methylbenzenesulfonic acid and the like.Somewhat elevated temperatures may be appropriate to enhance the rate ofthe reaction and in some cases the reaction may even be carried out atthe reflux temperature of the mixture. The water which is liberatedduring the condensation may be removed from the mixture by azeotropicaldistillation, distillation and the like methods. As representativeα-keto acids of formula (X) there may be named 2-oxopentanoic acid,2-oxoacetic acid, 2-oxopropanoic acid and the like acids. As suitableα-keto esters there may be named for example, ethyl 2-oxopropanoate,ethyl 4-methyl-2-oxopentanoate, ethyl 3-methyl-2-oxobutanoate, methylβ-oxobenzeneacetate, diethyl 2-methyl-3-oxo-1,4-butanedioate,diethyl-1,3-propanedioate and the like esters. As suitable halides theremay be named 2-oxopropanoyl chloride, dichloroacetyl chloride,diethoxyacethyl chloride.

In some instances the reaction of (VIII-b) with (X) first yields anintermediate of formula (XI-a) which may in situ or, if desired, afterisolating and purifying it, be cyclized by heating it in the presence ofan acid such as, for example, a carboxylic acid, e.g. acetic acid andthe like, a mineral acid such as, for example hydrochloric acid,sulfuric acid, or a sulfonic acid such as, for example, methanesulfonicacid, benzenesulfonic acid, 4-methylbenzenesulfonic acid and the like.##STR12## Alternatively compounds of formula (I-a-2-a) may be preparedby the reduction of an intermediate of formula (XI-b). ##STR13##

The reduction and cyclization of (XI-b) can conveniently be conducted bystirring the starting compound in a reaction inert solvent such as, forexample, an alkanol, e.g. methanol, ethanol, propanol and the like, asester, e.g. ethyl acetate, butylacetate and the like, an aromatichydrocarbon, e.g. benzene, methylbenzene and the like; a halogenatedhydrocarbon, e.g. chloromethane in the presence of hydrogen and anappropriate metal catalyst such as, for example, palladium-on-charcoal,Raney nickel and the like, optionally at an elevated temperature and/orpressure.

The compounds of formula (I) wherein Z is a radical of formula (a-2)wherein n is 1, said compounds being represented by formula (I-a-2-b)may be prepared by cyclizing an ortho-nitroanilide containing a suitableactivated methylenegroup of formula (XII-a). ##STR14##

The base promoted cyclization of (XII-a) can be conducted according toart-known cyclizing procedures as described in, for example, J. Chem.Soc., 1963, 2429; J. Med. Soc., 1966, 2285 and J. Org. Chem., 1968, 30,201 by stirring, and optionally heating the ortho-nitroanilide (XII-a)in a suitable solvent such as, for example water, an alcohol, e.g.methanol, ethanol and the like; a polar aprotic solvent, e.g. pyridineand the like; a ketone, e.g. propanone and the like; an aromatichydrocarbon e.g. benzene, dimethylbenzene and the like; a halogenatedhydrocarbon, e.g. trichloromethane, tetrachloromethane and the like; anether, e.g. tetrahydrofuran or a mixture of such solvents, in thepresence of an appropriate base. Suitable bases are for example, analkaline metal or an earth alkaline metal carbonate, hydrogen carbonate,hydroxide or hydride, e.g. sodium carbonate, sodium hydrogen carbonate,potassium carbonate, sodium hydroxide, sodium hydride and the like, oran organic base such as, for example, a tertiary amine, e.g.N-(1-methylethyl)-2-propanamine and the like. Depending on the reactionconditions and the nature of the activating group R⁴, the obtained3-substituted quinoxaline-N-oxide of formula (I-a-2-b) may be decomposedto give the corresponding unsubstituted N-oxide wherein R⁴ is hydrogen.

The compounds of formula (I-a-2-b) can also be prepared by cyclizing anortho anilide of formula (XII-b). ##STR15##

In (XII-b) P represents a suitable activating group such as, forexample, C₁₋₄ alkylcarbonyl, arylcarbonyl and the like. The basepromoted cyclization of (XII-b) can be carried out according similarprocedures as described hereinabove for the cyclization of (XII-a).Similar cyclization procedures are also outlined in J. Chem. Soc. 1963,p. 2431 and J. Chem. Soc. 1964, p. 2666.

The quinoxaline-2,3-diones of formula (I-a-3) can be prepared bycondensing an intermediate of formula (VIII-c) with oxalic acid (XIII)or a functional derivative thereof such as, for example an ester orhalide. ##STR16##

The condensation of (VIII-c) and (XIII) is conveniently carried out bymixing the reactants, optionally in a reaction inert solvent such as,for example, water; an alkanol, e.g. methanol, ethanol and the like; ahalogenated hydrocarbon, e.g. trichloromethane, dichloromethane and thelike; an ether, e.g. tetrahydrofuran; a dipolar aprotic solvent, e.g.N,N-dimethylformamide, N,N-dimethylacetamide, dimethylsulfoxide, anaromatic hydrocarbon, e.g. benzene, methylbenzene, dimethylbenzene andthe like; an ester, e.g. ethyl acetate or a mixture of such solventsoptionally in the presence of a carboxylic acid, e.g. acetic acid andthe like, a mineral acid such as, for example hydrochloric acid,sulfuric acid, or a sulfonic acid such as, for example, methanesulfonicacid, benzenesulfonic acid, 4-methylbenzenesulfonic acid and the like.In some instances the reaction may even be carried out in an excess ofcarboxylic acid, e.g. acetic acid and the like. Somewhat elevatedtemperatures may be appropriate to enhance the reaction and in somecases the reaction may even be carried out at the reflux temperature ofthe mixture. The water or acid which is liberated during condensationmay be removed by azeotropical distillation, distillation, complexation,salt formation and the like methods.

The compounds of formula (I) wherein Z is a radical of formula (a-4) maybe prepared by condensation of an ortho diamine of formula (VIII-d) withan α-halo acid of formula (XIV) or the ester form thereof ##STR17##

The above mentioned condensation can be carried out by stirring thereactants at an enhanced temperature in a suitable solvent such as, forexample, water; an alkanol, e.g. methanol, ethanol, propanol and thelike; an ether, e.g. 1,4-dioxane, 1,1'-oxybisethane, tetrahydrofuran andthe like; an ester, e.g. ethylacetate and the like; a halogenatedhydrocarbon, e.g. trichloromethane, tetrachloromethane and the like; adipolar aprotic solvent, e.g. N,N-dimethylformamide,N,N-dimethylacetamide, dimethylsulfoxide and the like, an aromatichydrocarbon, e.g. benzene, methylbenzene, dimethylbenzene and the like;a ketone, e.g. 2-propanone, 4-methyl-2-pentanone and the like andmixtures of such solvents. The addition of an appropriate base such as,for example, an alkali metal carbonate, hydrogen carbonate or hydroxide,e.g. sodium carbonate, sodium hydrogen carbonate, ammonium hydroxide oran organic base such as, for example, N,N-diethylethanamine and thelike, may be utilized to pick up the acid which is liberated during thecourse of the reaction.

Alternatively the α-ketotetrahydroquinoxalines of formula (I-a-4)wherein R⁷ is hydrogen, said compounds being represented by (I-a-4-a)may be prepared by the reduction of an appropriately substitutedortho-nitrophenylglycine of formula (XV). ##STR18##

The reduction of the ortho-nitrophenylglycine of formula (XV) canconveniently be conducted by stirring the starting material in areaction-inert solvent such as, for example, an alkanol, e.g. methanol,ethanol, propanol and the like, an ester, e.g. ethyl acetate,butylacetate and the like, an aromatic hydrocarbon, e.g. benzene,methylbenzene and the like; a halogenated hydrocarbon, e.g.chloromethane in the presence of hydrogen and an appropriate metalcatalyst such as, for example, palladium-on-charcoal, Raney nickel andthe like, optionally at an elevated temperature and/or pressure.Alternatively the reduction may be carried out with sodium dithionite inthe presence of acetic acid or in aqueous alkanol, e.g. an aqueousethanol solution.

Alternatively, some compounds of formula (I) may also be preparedaccording to procedures analogous to those described in the literaturefor the preparation of azoles by cyclizing an appropriate startingmaterial.

The compounds of formula (I-x) may also be prepared, for example, bycyclizing an intermediate of formula (XVI) and desulfurating the thusobtained intermediate of formula (XVII). ##STR19##

In formulae (XVI) and (XVII) R¹³ represents hydrogen or C₁₋₆ alkyl andR¹⁴ represents C₁₋₆ alkyl or both R¹⁴ taken together form a C₂₋₃alkanediyl radical.

Said cyclization reaction may conveniently be conducted by stirring andheating intermediate (XVI) in an aqueous acidic solvent, e.g. in aqueoushydrochloric or in concentrated sulfuric acid. The intermediate (XVII)may be desulfurated following art-known procedures, e.g., by treatmentwith Raney nickel in the presence of an alkanol, e.g. methanol, ethanoland the like, or by treatment with nitric acid, optionally in thepresence of sodium nitrite.

The compounds of formula (I-y) may be prepared from a hydrazinederivative of formula (XVIII) by reaction with s-triazine following theprocedures described in J. Org. Chem., 1956, 1037. ##STR20##

The intermediate hydrazine (XVIII) and the corresponding intermediateamine of formula Y--CH(NH₂)--Z (XIX) may also advantageously beconverted into azoles, wherein --X¹ ═X² -- is a bivalent radical offormula (x), (y) or (z), following the procedures described in U.S. Pat.No. 4,267,179, incorporated herein by reference.

The compounds of formula (I) can also be converted into each otherfollowing art-known group transformation procedures. The deoxygenationof the N-oxide of formula (I-a-2) can be carried out by stirring and, ifdesired, heating the starting compounds in a suitable solvent in thepresence of hydrogen or hydrazine and an appropriate metal catalyst suchas, for example, Raney nickel, Raney cobalt, platinum-on-charcoal,palladium-on-charcoal and the like metal catalysts. Suitable solventsare water, an alkanol, e.g. methanol, ethanol and the like, an ether,e.g. tetrahydrofuran and the like, and mixtures of such solvents wheretoan appropriate base has been added such as, for example, an alkali metalcarbonate, hydrogen carbonate or hydroxide, e.g. sodium carbonate,sodium hydrogen carbonate, sodium hydroxide and the like. Alternativelythe deoxygenation of the N-oxide of formula (I-a-2-b) may be carried outwith sodium dithionite in the presence of acetic acid or in an aqueousalkanol, e.g. an aqueous ethanol solution. It further proved possible toaccomplish the deoxygenation by stirring the N-oxide in the presence ofzinc and acetic acid.

The α-ketotetrahydroquinoxalines of formula (I-a-4) may also beconverted to a quinoxaline of formula (I-a-2) according to art-knowndehydrogenation procedures as described for example J. Chem. Soc., 1953,2816. For example, the dehydrogenation of the compounds of formula(I-a-4) can be carried out by heating the starting compound in anaqueous alkaline solution optionally in the presence of an appropriateoxidant such as, for example, peroxide, silver nitrate or manganese (IV)oxide.

Compounds of formula (I-a-2) wherein R³ is hydrogen and n is 0 may alsobe converted into the corresponding compounds of formula (I-a-1) whereinR¹ is halo by treatment with a halogenating agent such as, for example,thionyl chloride, phosphoryl chloride, pentachloro phosphorane, sulfurylchloride and the like. The thus obtained compounds wherein R¹ is halomay further be converted into quinoxalines of formula (I-a-1) wherein R¹is C₁₋₆ alkyloxy or mono or di(C₁₋₆ alkyl)amino by reacting the startingcompounds with an appropriate amine or alcohol, preferably an alkalimetal or earth alkaline metal salt of said alcohol. Some compounds offormula (I) may also be N-alkylated or N-aminated according to art knownprocedures.

Compounds of formula (I-a-5) can be obtained by reducing thecorresponding compounds of formula (I-a-1) with an appropriate reducingagent such as, for example, an alkali metal borohydride, e.g. lithium,potassium or preferably, sodium borohydride, sodium cyanoborohydride andthe like reducing agents in a reaction inert solvent.

A number of intermediates and starting materials in the foregoingpreparations are known compounds which may be prepared according toart-known methodologies of preparing said or similar compounds and someintermediates are new. A number of such preparation methods will bedescribed hereinafter in more detail.

Intermediates of formula (III), (V) and (VII) wherein Y is other thanhydrogen can be prepared from an appropriately substituted quinoxalinederivative of formula (XX) according to the following reaction sequence.##STR21##

The hydroxymethyl moiety of formula (XX) is converted into a formylmoiety with a suitable oxidizing agent, e.g. manganese (IV) oxide orpotassium permanganate, and subsequently reacted with a metal alkyl,e.g. methyllithium, butyllithium, metal aryl, e.g. phenyllithium, orwith a complex metal alkyl or aryl in a suitable solvent such as, forexample, an ether, e.g. tetrahydrofuran, 1,1'-oxybisethane and the liketo form the secundary alcohols. The desired intermediates of formula(III) may then be obtained by converting the alcohol function of theintermediate of formula (V) into an appropriate leaving group Wfollowing standard procedures as known in the art. For example, halidesare generally prepared by the reaction of (V) with an appropriatehalogenating agent such as, for example, thionyl chloride, sulfurylchloride, pentachlorophosphorane, pentabromophosphorane,phosphorylchloride, hydrochloric acid, hydrobromic acid and the likehalogenating agents. The intermediates of formula (III) wherein Y ishydrogen can be obtained directly from the intermediates of formula(VII) following the procedure described hereinabove for converting (V)into (III).

Some intermediates of formula (III) wherein Y is other than hydrogen mayalso be prepared by acylating an intermediate quinoxaline derivative offormula (XXI) with an appropriate acylating reagent (XXII) according toart-known Friedel-Crafts acylation reaction procedures, reducing theobtained ketone (VII-b) with an appropriate reductant, e.g. sodiumborohydride and the like in a suitable solvent such as an alcohol, e.g.methanol, ethanol; water or mixtures thereof with tetrahydrofuran andsubsequently converting the alcohol function into an appropriate leavinggroup W as described hereinbefore. ##STR22## Some intermediates offormula (III) may also be prepared by cyclizing an appropriatebenzaldehyde or ketone derivative of the general formula (XXIII)according to similar cyclization procedures as described hereinabove forthe synthesis of the compounds of formula (I-a-1), (I-a-2), (I-a-3),(I-a-4) or (I-a-5), reducing the thus obtained quinoxaline with anappropriate reductant, e.g. sodium borohydride, sodium cyanoborohydrideand the like reagents and subsequently converting the alcohol functionof (V) in an appropriate leaving group. Depending on the cyclizationprocedures it may be appropriate to protect the aldehyde or ketonefunction according to art-known procedures. ##STR23##

In (XXIII) the meanings of E¹ and E² are selected in such a manner toenable a cyclization reaction. For example, as appropriate intermediatesof formula (XXIII) there may be named the following: ##STR24##

More particular intermediates to prepare quinoxaline compounds may beprepared according the following procedures. The intermediates offormula (XI-b), (XII-a) and (XII-b) can conveniently be prepared byreacting an intermediate (XXV) with a carboxylic acid of formula(XXIV-a), (XXIV-b) or (XXIV-c) or a functional derivative thereof.##STR25##

Said functional derivative of (XXIV-a), (XXIV-b) or (XXIV-c) are meantto comprise the halide, a symmetrical or mixed anhydride, amide andester forms of (XXIV-a), (XXIV-b) or (XXIV-c). In the instance where R⁴represents a C₁₋₄ alkylcarbonyl group in formula (XXIV-b) the hydroxylgroup taken together with R⁴ may also form a reactive lactone such as,for example, 4-methylene-2-oxetanone. Functional derivatives may beprepared following art-known procedures, for example, by reacting thecarboxylic acid of formula (XXIV) with thionyl chloride, phosphoroustrichloride, polyphosphoric acid, phosphoryl chloride and the like, orby reacting the carboxylic acid of formula (XXIV) with an acyl halide,e.g. acetyl chloride, ethyl carbonochloridate and the like. Or theintermediates (XXV) and (XXIV) may be coupled in the presence of asuitable reagent capable of forming amides, e.g.dicyclohexylcarbodiimide, 1,1'-biscarbonyl[1H-imidazole],2-chloro-1-methylpyridinium iodide and the like.

Said amidation reactions may conveniently be carried out by stirring thereactants in a suitable reaction-inert solvent, such as, for example, ahalogenated hydrocarbon, e.g. dichloromethane, trichloromethane and thelike, an aromatic hydrocarbon, e.g. methylbenzene and the like, anether, e.g. 1,1'-oxybisethane, tetrahydrofuran and the like or a dipolaraprotic solvent, e.g. N,N-dimethylformamide, N,N-dimethylacetamide andthe like. The addition of a suitable base may be appropriate, inparticular a tertiary amine such as, N,N-diethylethanamine. The water,the alcohol or the acid which is liberated during the course of thereaction may be removed from the reaction mixture accordingmethodologies generally known in the art such as, for example,azeotropical distillation, complexation and salt formation.

Intermediates of formula (VIII) and (XXV) can easily be preparedaccording to procedures described in U.S. Pat. No. 4,859,684corresponding to EP-A-260,744 and U.S. Ser. No. 223,486 corresponding toEP-A-0,293,278 incorporated herein by reference for the process ofpreparing the intermediates of formula (VIII) and (XXV).

The intermediates of formula (XVI) can be prepared from an amine offormula (XXVII) by reaction with a reagent of formula (XXVI) andoptionally S-alkylating the thus obtained thiourea with a C₁₋₆alkylhalide. ##STR26##

The compounds of formula (I) and some of the intermediates in thisinvention have an asymmetric carbon atom in their structure. This chiralcenter may be present in a R- and a S-configuration, this R- andS-notation being in correspondence with the rules described in PureAppl. Chem., 1976, 45, 11-30. Pure stereochemically isomeric forms ofthe compounds of this invention may be obtained by the application ofart-known procedures. Diastereoisomers may be separated by physicalseparation methods such as selective crystallization and chromatographictechniques, e.g. counter current distribution, and enantiomers may beseparated from each other by the selective crystallization of theirdiastereomeric salts with optically active acids. Pure stereochemicallyisomeric forms may also be derived from the corresponding purestereochemically isomeric forms of the appropriate starting materials,provided that the reaction occurs stereospecifically.

The compounds of the present invention, their pharmaceuticallyacceptable acid addition salts and their possible stereochemicallyisomeric forms have useful pharmacological properties. For example, theysuppress the plasma elimination of retinoids, such as, alltrans-retinoicacid, 13-cis retinoic acid and their derivatives. The latter results inmore sustained/higher tissue concentrations of retinoic acid andimproved control of the differentiation and growth of various celltypes. In addition some compounds inhibit the formation of androgensfrom progestines and/or inhibit the action of the enzyme complexaromatase which catalyses the formation of estrogens from androgenicsteroids in mammals. A number of compounds also show an inhibitoryaction on the biosynthesis of thromboxane A₂.

Said property of the compounds of the invention to delay the metabolismof retinoic acid can easily be evidenced in various in vivo experiments.A particular test procedure is described hereinafter as the "Metabolismof endogenous or exogenously administered all-trans-retinoic acid" testand demonstrates the suppression of the plasma elimination of endogenousor exogenously administered all-trans-retinoic acid. As such, thecompounds of formula (I) can be used to control the rate of growth anddifferentiation of various cell types which effects are known to beaffected by retinoids. The ability of retinoids, such as,13-cis-retinoic acid, all-trans-retinoic acid and their derivatives tomodulate differentiation and proliferation in several cell types whetherthey are of epithelial or mesenchymal origin is extensively studied andreviewed in J. Clin. Chem. Clin, Biochem., 26, 479-488 (1983);Pharmacological Reviews 36, 935-1005, (1984), Arch. Dermatol. 117,160-180; (1981) and Journal of Medicinal Chemistry 25, 1269-1277,(1982).

In view of their capability to delay the metabolism of retinoic acid thecompounds can thus be used in the treatment of disorders which arecharacterized by an increased proliferation and/or abnormaldifferentiation of epithelial cells. In particular the compounds of theinvention can be used for treatment of carcinoma which is essentially aderailment of cellular differentiation, occurring in epithelial tissues.Other uses include, in addition to cancer treatment, the treatment of avariety of disorders of keratinization such as, for example, acne,psoriasis, lamellar ichthyosis, plantar warts, callosities, acanthosisnigricans, lichen planus, molluscum, melasma, corneal epithelialabrasion, geographic tongue, Fox-Fordyce disease, cutaneous metastaticmelanoma and keloids, epidermolytic hyperkeratosis, Darier's disease,pityriasis rubra pilaris, congenital ichthyosiform erythroderma,hyperkeratosis palmaris et plantaris, and similar diseases.

The anti-tumor activity may be demonstrated in several retinoicacid-sensitive and insensitive cell lines and solid tumors such as, forexample, in Ta3-Ha induced mamma tumors in female mice.

The inhibition of androgen and/or estrogen formation can be demonstratedby analyzing the effects of the compounds of the invention on theconversion of progestins into androgens in the presence of testicularmicrosomes or on the conversion of androstenedione into estrone andestradiol in the presence of human placental microsomes. The invivo-inhibition of androgen or estrogen formation can, for example, bedemonstrated by measuring the suppression of the plasma testosterone orestrogen concentration in dogs, rats or mice. A number of relevant testshave been described in EP-A-260,744 and EP-A-293,978 (U.S. Pat. No.4,943,574), both incorporated herein by reference. In view of theircapability to inhibit the biosynthesis of estrogens and/or androgens thecompounds can be used in the treatment of estrogen or androgen dependentdisorders such as, for example, breast cancer, endometriosis,endometrial cancer, polycystic ovarian disease, benign breast disease,prostatic cancer and hirsutism.

The beneficial effect of androgen inhibitors in these disorders,especially in the treatment of prostatic cancer, is described in, e.g.,Journal of Urology 132, 61-63 (1984). The beneficial effect of aromataseinhibitors in these disorders, especially in the treatment of breastcancer, is described in, e.g. Cancer Research, 42, Suppl. 8:3261s(1982).

In view of the usefulness of the subject compounds it is evident thatthe present invention provides a method for treating mammals sufferingfrom disorders which are characterized by an increased proliferationand/or abnormal differentiation of normal, preneoplastic or neoplasticcells, whether they are epithelial or mesenchymal; whether they are ofectodermal, endodermal or mesodermal origin; or whether they areestrogen dependent, androgen dependent or nonestrogen and nonandrogendependent. Said method comprises the systemic or topical administrationto the latter of an amount, effective to treat said disorders, of acompound of formula (I), a pharmaceutically acceptable acid-additionsalt, or a possible stereochemically isomeric form thereof. Inparticular the present invention provides a method in which the growthand differentiation in said normal, preneoplastic and neoplastic cellsis sensitive to the actions of retinoids.

Those of skill in treating disorders which are characterized by anexcessive proliferation and/or abnormal differentiation of tissues coulddetermine the effective amount from the test results presentedhereinafter. In general it is contemplated than an effective amountwould be from 0.001 mg/kg to 50 mg/kg body weight and more preferablyfrom 0.01 mg/kg to 10 mg/kg body weight.

The subject compounds may be formulated into various pharmaceuticalforms for administration purposes. As appropriate compositions there maybe cited all compositions usually employed for systemically or topicallyadministering drugs. To prepare the pharmaceutical compositions of thisinvention, an effective amount of the particular compound, optionally inacid-addition salt form, as the active ingredient is combined inintimate admixture with a pharmaceutically acceptable carrier, whichcarrier may take a wide variety of forms depending on the form ofpreparation desired for administration. These pharmaceuticalcompositions are desirable in unitary dosage form suitable,particularly, for administration orally, rectally, percutaneously, or byparenteral injection. For example, in preparing the compositions in oraldosage form, any of the usual pharmaceutical media may be employed suchas, for example, water, glycols, oils, alcohols and the like in the caseof oral liquid preparations such as suspensions, syrups, elixirs andsolutions; or solid carriers such as starches, sugars, kaolin,lubricants, binders, disintegrating agents and the like in the case ofpowders, pills, capsules, and tablets. Because of their ease inadministration, tablets and capsules represents the most advantageousoral dosage unit form, in which case solid pharmaceutical carriers areobviously employed. For parenteral compositions, the carrier willusually comprise sterile water, at least in large part, though otheringredients, for example, to aid solubility, may be included. Injectablesolutions, for example, may be prepared in which the carrier comprisessaline solution, glucose solution or a mixture of saline and glucosesolution. Injectable suspensions may also be prepared in which caseappropriate liquid carriers, suspending agents and the like may beemployed. Also included are solid form preparations which are intendedto be converted, shortly before use, to liquid form preparations. In thecompositions suitable for percutaneous administration, the carrieroptionally comprises a penetration enhancing agent and/or a suitablewetting agent, optionally combined with suitable additives of any naturein minor proportions, which additives do not introduce a significantdeleterious effect on the skin. As appropriate compositions for topicalapplication there may be cited all compositions usually employed fortopically administering drugs, e.g., creams, gellies, dressings,shampoos, tinctures, pastes, ointments, salves, powders and the like.Application of said compositions may be by aerosol e.g. with apropellent such as nitrogen carbon dioxide, a freon, or without apropellent such as a pump spray, drops, lotions, or a semisolid such asa thickened composition which can be applied by a swab. In particularcompositions, semisolid compositions such as salves, creams, gellies,ointments and the like will conveniently be used.

It is especially advantageous to formulate the aforementionedpharmaceutical compositions in dosage unit form for ease ofadministration and uniformity of dosage. Dosage unit form as used in thespecification and claims herein refers to physically discreate unitssuitable as unitary dosages, each unit containing a predeterminedquantity of active ingredient calculated to produce the desiredtherapeutic effect in association with the required pharmaceuticalcarrier. Examples of such dosage unit forms are tablets (includingscored or coated tablets), capsules, pills, powders packets, wafers,injectable solutions or suspensions, teaspoonfuls, tablespoonfuls andthe like, and segregated multiples thereof.

Other such compositions are preparations of the cosmetic type, such astoilet waters, packs, lotions, skin milks or milky lotions. Saidpreparations contain, besides the active ingredient, components usuallyemployed in such preparations. Examples of such components are oils,fats, waxes, surfactants, humectants, thickening agents, antioxidants,viscosity stabilizers, chelating agents, buffers, preservatives,perfumes, dyestuffs, lower alkanols, and the like. If desired, furtheringredients may be incorporated in the compositions, e.g.antiinflammatory agents, antibacterials, antifungals, disinfectants,vitamins, sunscreens, antibiotics, or other anti-acne agents. Examplesof oils comprise fats and oils such as olive oil and hydrogenated oils;waxes such as beeswax and lanolin; hydrocarbons such as liquid paraffin,ceresin, and squalane; fatty acids such as stearic acid and oleic acid;alcohols such as cetyl alcohol, stearyl alcohol, lanolin alcohol, andhexadecanol; and esters such as isopropyl myristate, isopropyl palmitateand butyl stearate. As examples of surfactants there may be citedanionic surfactants such as sodium stearate, sodium cetylsulfate,polyoxyethylene lauryl-ether phosphate, sodium N-acyl glutamate;cationic surfactants such as stearyldimethyl-benzylammonium chloride andstearyltrimethylammonium chloride; ampholytic surfac-tants such asalkylaminoethylglycine hydrochloride solutions and lecithin; andnonionic surfactants such as glycerin monostearate, sorbitanmonostearate, sucrose fatty acid esters, propylene glycol monostearate,polyoxyethylene oleylether, polyethylene glycol monostearate,polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fattyacid monoethanolamide, polyoxyethylene polyoxypropylene glycol (e.g. thematerials sold under the trademark "Pluronic"), polyoxyethylene castoroil, and polyoxyethylene lanolin. Examples of humectants includeglycerin, 1,3-butylene glycol, and propylene glycol; examples of loweralcohols include ethanol and isopropanol; examples of thickening agentsinclude xanthan gum, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyethylene glycol and sodium carboxymethyl cellulose;examples of antioxidants comprise butylated hydroxytoluene, butylatedhydroxyanisole, propyl gallate, citric acid and ethoxyquin; examples ofchelating agents include disodium edetate and ethanehydroxy diphosphate;examples of buffers comprise citric acid, sodium citrate, boric acid,borax, and disodium hydrogen phosphate; and examples of preservativesare methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dehydroaceticacid, salicylic acid and benzoic acid. For preparing ointments, creams,toilet waters, skin milks, and the like, typically from 0.01 to 10% inparticular from 0.1 to 5% and more in particular from 0.2 to 2.5% of theactive ingredient will be incorporated in said compositions. Inointments or creams, the carrier for example consists of 1 to 20%, inparticular 5 to 15% of a humectant, 0.1 to 10% in particular from 0.5 to5% of a thickener and water; or said carrier may consist of 70 to 99%,in particular 20 to 95% of a surfactant, and 0 to 20%, in particular 2.5to 15% of a fat; or 80 to 99.9% in particular 90 to 99% of a thickener;or 5 to 15% of a surfactant, 2-15% of a humectant, 0 to 80% of an oil,very small (<2%) amounts of preservative, colouring agent and/orperfume, and water. In a toilet water, the carrier for example consistsof 2 to 10% of a lower alcohol, 0.1 to 10% or in particular 0.5 to 1% ofa surfactant, 1 to 20%, in particular 3 to 7% of a humectant, 0 to 5% ofa buffer, water and small amounts (<2%) of preservative, dyestuff and/orperfume. In a skin milk, the carrier typically consists of 10-50% ofoil, 1 to 10% of surfactant, 50-80% of water and 0 to 3% of preservativeand/or perfume. In the afore-mentioned preparations, all % symbols referto weight by weight percentage. The humectant, surfactant, oil, etc. . .referred to in said preparations may be any such component used in thecosmetic arts but preferably will be one or more of the componentsmentioned hereinabove. Further, when in the above compositions one ormore of the components make up the major part of the composition, theother ingredients can evidently be not present at their indicatedmaximum concentration and therefore will make up the remainder of thecomposition.

Particular compositions for use in the method of the present inventionare those wherein the active ingredient is formulated inliposome-containing compositions. Liposomes are artificial vesiclesformed by amphiphatic molecules such as polar lipids, for example,phosphatidyl cholines, ethanolamines and serines, sphingomyelins,cardiolipins, plasmalogens, phosphatidic acids and cerebiosides.Liposomes are formed when suitable amphiphathic molecules are allowed toswell in water or aqueous solutions to form liquid crystals usually ofmultilayer structure comprised of many bilayers separated from eachother by aqueous material (also referred to as coarse liposomes).Another type of liposome known to be consisting of a single bilayerencapsulating aqueous material is referred to as a unilamellar vesicle.If water-soluble materials are included in the aqueous phase during theswelling of the lipids they become entrapped in the aqueous layerbetween the lipid bilayers.

In a further aspect of the invention there are provided particularpharmaceutical or cosmetical compositions which comprise an inertcarrier, an effective amount of a compound of formula (I) an acidaddition salt or a stereochemically isomeric form thereof and aneffective amount of a retinoic acid, a derivative thereof or astereochemically isomeric form thereof. Said retinoic acid containingcompositions are particularly useful for treating acne or for retardingthe effects of aging of the skin and generally improve the quality ofthe skin, particularly human facial skin. A pharmaceutical or cosmeticalcomposition containing retinoic acid or a derivative thereof as theactive ingredient in intimate admixture with a dermatologicallyacceptable carrier can be prepared according to conventional compoundingtechniques, such as those known for topical application of retinoic acidand its derivatives. Conventional pharmaceutical compounding techniquesfor topical application of retinoic acid are described for example in,U.S. Pat. Nos. 3,906,108 and 4,247,547, which are incorporated herein byreference. Preferred composition for topical application are in form ofa cream, ointment or lotion comprising from 0.005 to 0.5% (particularlyfrom 0.01 to 0.1%) alltrans-retinoic acid, 13-cis-retinoic acid or aderivative thereof and from 0.1 to 5% of a compound of formula (I) and,a dermatologically acceptable acid addition salt thereof or astereochemically isomeric form thereof, in a semi-solid or liquiddiluent or carrier. These preferred compositions should preferably benon-irritating and as far as possible they should be odorless andnon-toxic. For convenience in applying to the skin, the compositionusually contain, besides water or an organic solvent, several of certainorganic emollients, emulsifiers for the aqueous and/or non aqueousphases of the compositions, wetting agents preservatives and agents thatfacilitate the penetration and remainence of the active agents in theskin.

The following examples are intended to illustrate and not to limit thescope of the present invention. Unless otherwise stated all partstherein are by weight.

EXPERIMENTAL PART A. PREPARATION OF THE INTERMEDIATES EXAMPLE 1

A mixture of 10 parts of 5-methylquinoxaline, 10 parts of1,3-dibromo-5,5-dimethylimidazolidine-2,4-dione, 1.7 parts ofbenzenecarboperoxoic acid and 318 parts of tetrachloromethane wasstirred for 16 hours at reflux temperature under 2 lamps of 250 Watt.The reaction mixture was cooled and the organic layer was decanted. Theproduct was filtered off and dried, yielding 15.5 parts (100%) of5-(bromomethyl)quinoxaline (interm. 1).

EXAMPLE 2

(a) To a stirred solution of 30 parts of (3,4-diaminophenyl)phenylmethanone in 240 parts of methanol were added 30 parts of anethanedial solution in water 40%. The reaction mixture was stirred for 3hours at reflux temperature. After cooling to room temperature, theprecipitated product was filtered off, washed with methanol and dried,yielding 20 parts (59.3%) of phenyl (6-quinoxalinyl)methanone; mp. 120°C. (interm. 2).

(b) To a stirred and cooled (5° C.) solution of 20 parts of intermediate2, namely phenyl (6-quinoxalinyl)methanone in 160 parts of methanol wereadded portionwise 3.2 parts of sodium tetrahydroborate. Upon completion,the reaction mixture was poured into water and the product was extractedwith dichloromethane. The extract was washed with water, dried, filteredand evaporated to dry, yielding 20 parts (100%) ofα-phenyl-6-quinoxalinemethanol as an oily residue (interm. 3).

(c) To a stirred and cooled (0° C.) mixture of 12 parts of intermediate3, namely α-phenyl-6-quinoxalinemethanol, 213 parts of dichloromethaneand 15.4 parts of N,N-diethylethanamine was added a solution of 8.8parts of methanesulfonyl chloride in 26.6 parts of dichloromethane undernitrogen atmosphere. After stirring overnight at room temperature, thereaction mixture was evaporated, yielding 54 parts (100%) ofα-phenyl-6-quinoxalinemethanol methanesulfonate (ester) as an oilyresidue (interm. 4).

EXAMPLE 3

(a) To a stirred mixture of 6.9 parts of 3,4-diaminobenzenemethanol, 1part of N,N-diethylethanamine and 75 parts of water were added 2.9 partsof a solution of ethanedial in water 40% at about 55° C. The whole wasstirred for 1 hour at 55°-60° C. The solvent was evaporated and theresidue was purified by column chromatography over silica gel using amixture of trichloromethane and methanol (95:5 by volume) as eluent. Thepure fractions were collected and the eluent was evaporated. The residuewas converted into the hydrochloride salt in 2-propanol and ethanol. Theproduct was filtered off and dried, yielding 6.5 parts (66.1%) of6-quinoxalinemethanol monohydrochloride; mp. >300° C. (interm. 5).

(b) To a stirred solution of 10 parts of intermediate 5, namely6-quinoxalinemethanol in 133 parts of dichloromethane were added 20parts of manganese(IV) oxide. After stirring for 3 hours at roomtemperature, the reaction mixture was filtered and the filtrate wasevaporated, yielding 6.6 parts (67.3%) of 6-quinoxalinecarboxaldehyde;mp. 134° C. (interm. 6).

EXAMPLE 4

(a) To a stirred and refluxed Grignard complex previously preparedstarting from 55.1 parts of 1-bromopropane, 10.9 parts of magnesium andtetrahydrofuran was added a solution of 25 parts ofN-(4-formylphenyl)acetamide in 225 parts of dry tetrahydrofuran. Afterstirring for 1 hour at room temperature, the reaction mixture was pouredinto ice water and a saturated ammonium chloride solution. The organiclayer was decanted (and set aside) and the remaining phase was extractedwith ethyl acetate. The combined organic layers were dried, filtered andevaporated. The residue was purified by column chromatography oversilica gel using a mixture of trichloromethane and methanol (98:2 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated, yielding 20 parts (64.3%) ofN-[4-(1-hydroxybutyl)phenyl]acetamide as a residue (interm. 7).

(b) A mixture of 10 parts of intermediate 7, namelyN-[4-(1-hydroxybutyl)phenyl]acetamide, 16.2 parts of1,1'-carbonylbis[1H-imidazole] and 135 parts of tetrahydrofuran wasstirred for 17 hours at room temperature. The reaction mixture wasevaporated and the residue was taken up in trichloromethane. The organicphase was washed with a potassium carbonate solution 10% in water,dried, filtered and evaporated. The residue was purified by columnchromatography (HPLC) over silica gel using a mixture oftrichloromethane and methanol (95:5 by volume) as eluent. The purefractions were collected and the eluent was evaporated, yielding 5.8parts (45.0%) of N-[4-[1-(1H-imidazol-1-yl)butyl]phenyl]acetamide; mp.186° C. (interm. 8);

(c) To a stirred and cooled (0° C.) mixture of 2.57 parts ofintermediate 8, namely N-[4-[1-(1H-imidazol-1-yl)butyl]phenyl]acetamideand 23.0 parts of concentrated sulfuric acid were added portionwise 1.01parts of potassium nitrate. Upon complete addition, stirring wascontinued for 30 minutes at 0° C. The reaction mixture was poured intocrushed ice and treated with ammonium hydroxide to pH 10. The productwas extracted with trichloromethane. The extract was dried, filtered andevaporated, yielding 3 parts (99.4%) ofN-[4-[1-(1H-imidazol-1-yl)butyl]-2-nitrophenyl]acetamide as a residue(interm. 9).

(d) A mixture of 11.5 parts of intermediate 9, namelyN-[4-[1-(1H-imidazol-1-yl)butyl]-2-nitrophenyl]acetamide and 150 partsof a hydrochloric acid solution 3N was stirred for 3 hours at refluxtemperature. The reaction mixture was poured into crushed ice and thewhole was neutralized with concentrated ammonium hydroxide. The productwas extracted with dichloromethane. The extract was dried, filtered andevaporated, yielding 8.8 parts (88.9%) of4-[1-(1H-imidazol-1-yl)butyl]-2-nitrobenzenamine as a residue (interm.10).

In a similar manner there were also prepared:

4-[1-(1H-imidazol-1-yl)propyl]-2-nitrobenzenamine as a residue (interm.11);

4-[1-(1H-imidazol-1-yl)-3-methylbutyl]-2-nitrobenzenamine as a residue(interm. 12); and

4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrobenzenamine (interm. 13).

EXAMPLE 5

(a) To a stirred and cooled mixture (ice bath, 0° C.) of 30 parts of1-(4-amino-3-nitrophenyl)-2-methyl-1-propanone and 390 parts ofdichloromethane were added dropwise 33 parts of acetyl chloride. Uponcomplete addition, the reaction mixture was stirred for 12 hours at roomtemperature. The whole was poured into water and after the addition ofsodium carbonate, stirring was continued for 15 minutes. The separatedorganic layer was dried, filtered and evaporated, yielding 36 parts(100%) of N-[4-(2-methyl-1-oxopropyl)-2-nitrophenyl]acetamide (interm.14).

(b) To a stirred and cooled (ice water, 10° C.) solution of 30 parts ofintermediate 14, namelyN-[4-(2-methyl-1-oxopropyl)-2-nitrophenyl]acetamide in 240 parts ofmethanol were added portionwise 4.5 parts of sodium tetrahydroborate.Upon completion, stirring was continued for 1 hour. The reaction mixturewas evaporated and the residue was extracted with dichloromethane (3×104parts). The combined extracts were washed with water, dried, filteredand evaporated, yielding 32 parts (100%) ofN-[4-(1-hydroxy-2-methylpropyl)-2-nitrophenyl]acetamide (interm. 15).

(c) To a stirred solution of 36 parts of intermediate 15, namelyN-[4-(1-hydroxy-2-methylpropyl)-2-nitrophenyl]acetamide and 390 parts ofdichloromethane were added 35.0 parts of N,N-diethylethanamine. Aftercooling to 0° C., 20.0 parts of methane-sulfonyl chloride were addeddropwise to the previous mixture. Upon completion, stirring wascontinued for 12 hours at room temperature. The whole was poured intowater and sodium carbonate was added while stirring. The product wasextracted with dichloromethane. The extract was dried, filtered andevaporated, yielding 39 parts (100%) ofN-[4-(1-chloro-2-methylpropyl)-2-nitrophenyl]acetamide (interm. 16).

(d) A mixture of 40 parts of intermediate 16, namelyN-[4-(1-chloro-2-methylpropyl)-2-nitrophenyl]acetamide, 51 parts of1H-1,2-4-triazole, 50 parts of potassium carbonate and 400 parts ofacetonitrile was stirred for 2 hours at reflux temperature. Aftercooling, the whole was evaporated to dry and the residue was taken up in300 parts of water. The product was extracted with dichloromethane. Theextract was dried, filtered and evaporated. The residue was purified bycolumn chromatography over silica gel using a mixture of dichloromethaneand methanol (98:2 by volume) as eluent. The pure fractions werecollected and the eluent was evaporated, yielding 22 parts (49.0%) ofN-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]acetamideas an oil (interm. 17).

(e) A mixture of 20 parts of intermediate 17, namelyN-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]acetamideand 200 parts of a hydrochloric acid solution 2N was stirred for 12hours at room temperature. The reaction mixture was poured into 500parts of water and the whole was neutralized with a concentratedpotassium carbonate solution. The product was extracted withdichloromethane (3×130 parts). The combined extracts were dried,filtered and evaporated to dry. The residue was crystallized from2,2'-oxybispropane, yielding 18.5 parts (97.7%) of4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrobenzenamine; mp.206° C. (interm. 18).

In a similar manner there were also prepared:

                  TABLE 1                                                         ______________________________________                                         ##STR27##                                                                    Int. No. X.sup.1X.sup.2                                                                            R         Y                                              ______________________________________                                        19       CHN         H         C.sub.6 H.sub.5                                20       CHCH        2-CH.sub.3                                                                              i-C.sub.3 H.sub.7                              21       CHN         H         C.sub.2 H.sub.5                                22       CHN         H         CH.sub.2CH(CH.sub.3).sub.2                     23       NCH         H         i-C.sub.3 H.sub.7                              24       CHN         H         C.sub.4 H.sub.9                                25       CHN         H         C.sub.3 H.sub.7                                ______________________________________                                    

EXAMPLE 6

(a) 1.61 Parts of sodium tetrahydroborate were added dropwise to astirred solution of 11 parts of (4-amino-3-nitrophenyl)(2-fluorophenyl)methanone in 120 parts of methanol. Upon completeaddition, stirring was continued for 1 hour at room temperature. Thereaction mixture was poured into water and the product was extractedthree times with 75 parts of trichloromethane. The combined extractswere dried, filtered and evaporated, yielding 11.3 parts (100%) of4-amino-α-(2-fluorophenyl)-3-nitrobenzenemethanol as a residue (interm.26).

(b) A mixture of 11.1 parts of intermediate 26, namely4-amino-α-(2-fluorophenyl)-3-nitrobenzenemethanol, 13.7 parts of1,1'-carbonylbis[1H-imidazole] and 90 parts of N,N-dimethylformamide wasstirred for 12 hours at room temperature. After evaporation to dry, theresidue was taken up in dichloromethane. The organic phase was washedwith 50 parts of water, dried, filtered and evaporated. The residue waspurified by column chromatography over silica gel using a mixture ofdichloromethane and methanol (95:5 by volume) as eluent. The purefractions were collected and the eluent was evaporated, yielding 6.5parts (49.5%) of4-[(2-fluorophenyl)(1H-imidazol-1-yl)methyl]-2-nitrobenzenamine; mp.176° C. (interm. 27).

In a similar manner there were also prepared:

                  TABLE 2                                                         ______________________________________                                         ##STR28##                                                                    Int. No.   Y              physical data                                       ______________________________________                                        28         3-pyridinyl    164.1° C.                                    29         phenyl         --                                                  30         2-thienyl      --                                                  31         4-fluorophenyl 147.4° C.                                    32         3-chlorophenyl --                                                  33         3,4-dichlorophenyl                                                                           152° C.                                      34         cyclopropyl    --                                                  35         4-methoxyphenyl                                                                              --                                                  36         butyl          --                                                  37         3-fluorophenyl --                                                  38         2-chlorophenyl --                                                  39         4-methylphenyl --                                                  40         3-CF.sub.3 -phenyl                                                                           --                                                  41         4-chlorophenyl --                                                  42         cyclohexyl     --                                                  43         cyclopentyl    --                                                  44         4-[CH(CH.sub.3).sub.2 ]-phenyl                                                               --                                                  ______________________________________                                    

EXAMPLE 7

(a) To a stirred solution of 30 parts of1-(4-chloro-3-nitrophenyl)-2-methyl-1-propanone in 240 parts of methanolwas added a solution of 20 parts of methanamine in 160 parts ofmethanol. After stirring for 12 hours at 60° C., the reaction mixturewas evaporated to dry, yielding 30 parts (100%) of2-methyl-1-[4-(methylamino)-3-nitrophenyl]-1-propanone as a residue(interm. 45).

(b) To a stirred solution of 30 parts of intermediate 45, namely2-methyl-1-[4-(methylamino)-3-nitrophenyl]-1-propanone in 320 parts ofmethanol were added dropwise 15 parts of sodium tetrahydroborate (thetemperature was kept at 20° C.). Upon complete addition, stirring wascontinued for 30 minutes at room temperature. The reaction mixture waspoured into water and the product was extracted with trichloromethane.The extract was dried, filtered and evaporated to dry, yielding 30 parts(100%) of 4-(methylamino)-α-(1-methylethyl)-3-nitrobenzenemethanol as anoily residue (interm. 46).

(c) To a stirred solution of 30 parts of intermediate 46, namely4-(methylamino)-α-(1-methylethyl)-3-nitrobenzenemethanol in 270 parts ofdry tetrahydrofuran were added 43.4 parts of1,1'-carbonylbis[1H-imidazole]. After stirring for 24 hours at roomtemperature, the reaction mixture was evaporated to dry. The residue wastaken up in trichloromethane and a potassium carbonate solution 10%. Theseparated organic layer was dried, filtered and evaporated to dry. Theresidue was purified by column chromatography over silica gel using amixture of trichloromethane and methanol (99:1 by volume) as eluent. Thepure fractions were collected and the eluent was evaporated, yielding 15parts (40.9%) of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-N-methyl-2-nitrobenzenamine as aresidue (interm. 47).

EXAMPLE 8

To a stirred and cooled (0° C.) solution of 7 parts of4-[1-(1H-imidazol-1-yl)propyl]-2-nitrobenzenamine in 126 parts of1,2-dichloroethane were added 9.6 parts of 2-methylbenzeneacetylchloride. After stirring for 12 hours at room temperature, the reactionmixture was poured into ice water and the product was extracted withdichloromethane. The extract was dried, filtered and evaporated invacuo. The oily residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (98:2 byvolume) as eluent. The eluent of the desired fraction was evaporated,yielding 9.6 parts (89.3%) ofN-[4-[1-(1H-imidazol-1-yl)propyl]-2-nitrophenyl]-2-methylbenzeneacetamide;mp. 122° C. (interm. 48).

In a similar manner there were also prepared:

                                      TABLE 3                                     __________________________________________________________________________     ##STR29##                                                                    Int. No.                                                                           X.sup.1X.sup.2                                                                        R   Y        Z          mp.                                      __________________________________________________________________________    49   CHN     H   C.sub.6 H.sub.5                                                                        C.sub.6 H.sub.5                                                                          --                                       50   CHCH    H   i-C.sub.3 H.sub.7                                                                      2-FC.sub.6 H.sub.4                                                                       --                                       51   CHCH    H   i-C.sub.3 H.sub.7                                                                      4-CH.sub.3C.sub.6 H.sub.4                                                                114° C.                           52   CHCH    2-CH.sub.3                                                                        i-C.sub.3 H.sub.7                                                                      C.sub.6 H.sub.5                                                                          --                                       53   CHCH    H   i-C.sub.3 H.sub.7                                                                      4-BrC.sub.6 H.sub.4                                                                      --                                       54   CHCH    H   i-C.sub.3 H.sub.7                                                                      3,4-F.sub.2C.sub.6 H.sub.3                                                               --                                       55   CHCH    H   i-C.sub.3 H.sub.7                                                                      4-OCH.sub.3C.sub.6 H.sub.4                                                               --                                       56   CHCH    H   2,4-Cl.sub.2C.sub.6 H.sub.3                                                            C.sub.6 H.sub.5                                                                          --                                       57   CHCH    H   i-C.sub.3 H.sub.7                                                                      3,4-(OCH.sub.3).sub.2C.sub.6 H.sub.3                                                     --                                       58   CHCH    H   i-C.sub.3 H.sub.7                                                                      2,4-Cl.sub.2C.sub.6 H.sub.3                                                              --                                       59   CHCH    H   i-C.sub.3 H.sub.7                                                                      2-naphthalenyl                                                                           144° C.                           60   CHCH    H   i-C.sub.3 H.sub.7                                                                      3,4,5-(OCH.sub.3).sub.3C.sub.6 H.sub.2                                                   --                                       61   CHCH    H   i-C.sub.3 H.sub.7                                                                      2-thienyl  --                                       62   CHCH    H   i-C.sub.3 H.sub.7                                                                      2-OCH.sub.3C.sub.6 H.sub.4                                                               --                                       63   CHCH    H   i-C.sub.3 H.sub.7                                                                      1-naphthalenyl                                                                           --                                       64   CHCH    H   i-C.sub.3 H.sub.7                                                                      2-ClC.sub.6 H.sub.4                                                                      --                                       65   CHCH    H   i-C.sub.3 H.sub.7                                                                      3-OHC.sub.6 H.sub.4                                                                      --                                       66   CHCH    H   i-C.sub.3 H.sub.7                                                                      3-BrC.sub.6 H.sub. 4                                                                     --                                       67   CHCH    H   i-C.sub.3 H.sub.7                                                                      3-thienyl  --                                       68   CHN     H   i-C.sub.3 H.sub.7                                                                      2-thienyl  --                                       69   CHCH    H   i-C.sub.3 H.sub.7                                                                      2Cl,6FC.sub.6 H.sub.3                                                                    --                                       70   CHCH    H   i-C.sub.3 H.sub.7                                                                      3Br,4OHC.sub.6 H.sub.3                                                                   --                                       71   CHN     H   C.sub.2 H.sub.5                                                                        C.sub.6 H.sub.5                                                                          --                                       72   CHN     H   CH.sub.2CH(CH.sub.3).sub.2                                                             C.sub.6 H.sub.5                                                                          --                                       73   NCH     H   i-C.sub.3 H.sub.7                                                                      4-FC.sub.6 H.sub.4                                                                       --                                       74   CHN     H   C.sub.4 H.sub.9                                                                        3-FC.sub.6 H.sub.4                                                                       --                                       75   CHCH    H   i-C.sub.3 H.sub.7                                                                      3Cl,4OHC.sub.6 H.sub.3                                                                   --                                       76   NCH     H   i-C.sub.3 H.sub.7                                                                      C.sub.6 H.sub.5                                                                          --                                       77   CHN     H   C.sub.3 H.sub.7                                                                        2-CH.sub.3C.sub.6 H.sub.4                                                                --                                       78   NCH     H   i-C.sub.3 H.sub.7                                                                      3-ClC.sub.6 H.sub.4                                                                      --                                       79   CHN     H   CH.sub.2CH(CH.sub.3).sub.2                                                             2-CH.sub.3C.sub.6 H.sub.4                                                                --                                       80   CHN     H   C.sub.2 H.sub.5                                                                        2-CH.sub.3C.sub.6 H.sub.4                                                                --                                       81   NCH     H   i-C.sub.3 H.sub.7                                                                      3-FC.sub.6 H.sub.4                                                                       --                                       82   CHN     H   i-C.sub.3 H.sub.7                                                                      2-FC.sub.6 H.sub.4                                                                       --                                       83   CHN     H   C.sub.4 H.sub.9                                                                        2-CH.sub.3C.sub.6 H.sub.4                                                                --                                       84   CHN     H   C.sub.3 H.sub.7                                                                        3-FC.sub.6 H.sub.4                                                                       --                                       __________________________________________________________________________

EXAMPLE 9

To a stirred and cooled (0° C.) mixture of 8 parts of4-(1H-imidazol-1-yl)methyl]-2-nitrobenzenamine and 106 parts ofdichloromethane were added 3.4 ml of 4-methylene-2-oxetanone. Afterstirring for 1 hour at 0° C., another portion of 3.4 ml of4-methylene-2-oxetanone was added and stirring was continued for 1 hourat this low temperature. The reaction mixture was diluted with 8 partsof methanol and evaporated to dry. The residue was crystallized from2-propanone, yielding 7.6 parts (68.7%) ofN-[4-(1H-imidazol-1-yl)methyl]-2-nitrophenyl]-3-oxobutanamide; mp. 172°C. (interm. 85).

In a similar manner there were also prepared:

                  TABLE 4                                                         ______________________________________                                         ##STR30##                                                                    Int. No.      Y          mp.                                                  ______________________________________                                        86            i-C.sub.3 H.sub.7                                                                         85° C.                                       87            3-ClC.sub.6 H.sub.4                                                                      --                                                   88            c-C.sub.6 H.sub.11                                                                       --                                                   89            4-ClC.sub.6 H.sub.4                                                                      --                                                   90            c-C.sub.5 H.sub.9                                                                        --                                                   91            CH.sub.3   134° C.                                       92            C.sub.2 H.sub.5                                                                          --                                                   93            4-FC.sub.6 H.sub.4                                                                       --                                                   94            2-FC.sub.6 H.sub.4                                                                       --                                                   95            3-FC.sub.6 H.sub.4                                                                       --                                                   ______________________________________                                    

EXAMPLE 10

A solution of 10 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrobenzenamine, 20 parts ofethyl β-oxobenzenepropanoate and 174 parts of benzene was stirred for 36hours at reflux temperature. After cooling, the reaction mixture wasevaporated. The residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (98:2 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated, yielding 7 parts (44.8%) ofN-[4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrophenyl]-β-oxobenzenepropanamide;mp. 106° C. (interm. 96).

In a similar manner there were also prepared:

N-[4-[1-(1H-imidazol-1-yl)ethyl]-2-nitrophenyl]-β-oxobenzenepropanamide;mp. 172° C. (interm. 97);

N-[4-(1H-imidazol-1-ylmethyl)-2-nitrophenyl]-β-oxobenzenepropanamide;mp. 98° C. (interm. 98); and

N-[4-[(1H-imidazol-1-yl)phenylmethyl]-2-nitrophenyl]-β-oxobenzenepropanamide(interm. 99).

EXAMPLE 11

To a stirred solution of 13 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrobenzenamine in 195 partsof dichloromethane was added a solution of 19 parts of3-chlorobenzeneacetyl chloride in 65 parts of dichloromethane. Afterstirring for 4 hours at room temperature, 10.1 parts ofN,N-diethylethanamine were added. The reaction mixture was washed withwater, dried, filtered and evaporated, yielding 39 parts (100%) of3-chloro-N-[4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrophenyl]benzeneacetamideas an oily residue (interm. 100).

In a similar manner there were also prepared:

                  TABLE 5                                                         ______________________________________                                         ##STR31##                                                                    Int. No.  X.sup.1X.sup.2                                                                             Y           X                                          ______________________________________                                        101       CHCH         i-C.sub.3 H.sub.7                                                                         4-Cl                                       102       CHCH         i-C.sub.3 H.sub.7                                                                         4-F                                        103       CHN          i-C.sub.3 H.sub.7                                                                         H                                          104       CHCH         C.sub.4 H.sub.9                                                                           H                                          105       CHCH         i-C.sub.3 H.sub.7                                                                         3-F                                        106       CHCH         4-ClC.sub.6 H.sub.4                                                                       H                                          107       CHCH         3-ClC.sub.6 H.sub.4                                                                       H                                          108       CHCH         i-C.sub.3 H.sub.7                                                                         2-CH.sub.3                                 109       CHCH         i-C.sub.3 H.sub.7                                                                         3-OCH.sub.3                                110       CHCH         i-C.sub.3 H.sub.7                                                                         3-CH.sub.3                                 111       CHCH         i-C.sub.3 H.sub.7                                                                         4-OC.sub.2 H.sub.5                         ______________________________________                                    

EXAMPLE 12

To a stirred and cooled (5° C.) solution of 10 parts of4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrobenzenamine and 6.7parts of pyridine in 195 parts of dichloromethane was added a solutionof 14.4 parts of 4-chlorobenzeneacetyl chloride in 39 parts ofdichloromethane under nitrogen atmosphere. After stirring overnight atroom temperature, the reaction mixture was washed with water, dried,filtered and evaporated. The residue was purified by columnchromatography over silica gel using a mixture of dichloromethane andmethanol (99:1 by volume) as eluent. The eluent of the desired fractionwas evaporated, yielding 11 parts (69.9%) of4-chloro-N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]benzeneacetamide(interm. 112).

In a similar manner there were also prepared:

4-fluoro-N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]benzeneacetamide(interm. 113);

3-fluoro-N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]benzeneacetamide(interm. 114);

N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]-3-thiopheneacetamide(interm. 115); and

3-chloro-N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]benzeneacetamide(interm. 116).

EXAMPLE 13

A mixture of 31 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrobenzenamine and 240 partsof ethanol was hydrogenated at 0.5.10⁵ Pa in a Parr apparatus and atroom temperature with 30 parts of Raney nickel catalyst. After thecalculated amount of hydrogen was taken up, the catalyst was filteredoff over diatomaceous earth and the filtrate was evaporated, yielding27.4 parts (100%) of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-1,2-benzenediamine as a residue(interm. 117).

In a similar manner there were also prepared:

                  TABLE 6                                                         ______________________________________                                         ##STR32##                                                                    Int. No.  X.sup.1X.sup.2                                                                            Z        Y                                              ______________________________________                                        118       CHCH        H        C.sub.6 H.sub.5                                119       CHCH        H        3-pyridinyl                                    120       CHCH        H        2-thienyl                                      121       CHCH        H        4-FC.sub.6 H.sub.4                             122       CHCH        H        3-ClC.sub.6 H.sub.4                            123       CHCH        H        c-C.sub.3 H.sub.5                              124       CHCH        H        CH.sub.3                                       125       CHCH        H        C.sub.4 H.sub.9                                126       CHCH        H        C.sub.2 H.sub.5                                127       CHCH        H        CH.sub.2CH(CH.sub.3).sub.2                     128       CHCH        CH.sub.3 i-C.sub.3 H.sub.7                              129       CHCH        H        C.sub.3 H.sub.7                                130       CHCH        H        3-FC.sub.6 H.sub.4                             131       CHCH        H        2-ClC.sub.6 H.sub.4                            132       CHCH        H        2-FC.sub.6 H.sub.4                             132       CHCH        H        4-CH.sub.3C.sub.6 H.sub.4                      133       CHCH        H        3-CF.sub.3C.sub.6 H.sub.4                      134       CHCH        H        4-ClC.sub.6 H.sub.4                            135       CHN         H        i-C.sub.3 H.sub.7                              136       CHCH        H        c-C.sub.6 H.sub.11                             137       CHCH        H        c-C.sub.5 H.sub.9                              138       CHCH        H        4-(i-C.sub.3 H.sub.7)C.sub.6 H.sub.4           ______________________________________                                    

B. PREPARATION OF THE FINAL COMPOUNDS EXAMPLE 14

A mixture of 15.5 parts of 5-(bromomethyl)quinoxaline, 23.5 parts of1H-imidazole and 160 parts of acetonitrile was stirred for 1.5 hours atreflux temperature. The reaction mixture was evaporated and the residuewas taken up in water. The product was extracted three times with 65parts of dichloromethane. The combined extracts were dried, filtered andevaporated. The residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (95:5 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated. The residue was crystallized from a mixture of 2-propanoneand 2,2'-oxybispropane. The product was filtered off and dried, yielding3 parts (20.5%) of 5-(1H-imidazol-1-yl-methyl)quinoxaline; mp. 121.2° C.(comp. 41).

EXAMPLE 15

A mixture of 10.4 parts of α-phenyl-6-quinoxalinemethanolmethanesulfonate(ester), 12 parts of 1H-1,2,4-triazole and 79 parts ofacetonitrile was stirred overnight at reflux temperature. The reactionmixture was evaporated and the residue was extracted with ethyl acetate.The extract was dried, filtered and evaporated. The residue was purifiedby column chromatography (silica gel; CH₂ Cl₂ /CH₃ OH 96:4). The eluentof the desired fraction was evaporated and the residue was crystallizedfrom a mixture of 2-propanol and 2,2'-oxybispropane, yielding 0.9 parts(9.5%) of 6-[phenyl(4H-1,2,4-triazol-4-yl)methyl]quinoxaline; mp. 98.1°C. (comp. 50).

EXAMPLE 16

A mixture of 7.8 parts of 6-quinoxalinecarboxaldehyde and 24.3 parts of1,1'-carbonylbis-1H-imidazole was stirred for 1 hour at 100° C. Aftercooling, the reaction mixture was partitioned between water and ethylacetate. The organic layer was dried, filtered and evaporated. Theresidue was purified by column chromatography (silica gel; CH₂ Cl₂ /CH₃OH/NH₄ OH 85:15:1). The eluent of the desired fraction was evaporatedand the residue was converted into the 4-methylbenzenesulfonate(1:2)salt in 2-propanone, yielding 7 parts (23.0%) of6-[di(1H-imidazol-1-yl)methyl]quinoxaline 4-methylbenzenesulfonate(1:2);mp. 240.3° C. (comp. 51).

EXAMPLE 17

(a) A mixture of 3.76 parts of4-(1H-imidazol-1-ylmethyl)-1,2-benzenediamine, 4.5 parts ofdiphenylethanedione and 80 parts of ethanol was stirred for 4 hours atreflux temperature. The reaction mixture was concentrated and theconcentrate was purified by column chromatography over silica gel usinga mixture of trichloromethane and methanol (95:5 by volume) as eluent.The pure fractions were collected and the eluent was evaporated. Theresidue was crystallized from a mixture of 1,1'-oxybisethane andethanol. The product was filtered off and dried, yielding 4 parts (55%)of 6-(1H-imidazol-1-ylmethyl)-2,3-diphenylquinoxaline; mp. 159.3° C.(comp. 8).

(b) 6-[(1H-imidazol-1-yl)(phenyl)methyl]quinoxaline; mp. 126.8° C.(comp. 5) was prepared following substantially the same procedures as inexample 17a except that ethanedial was used in place ofdiphenylethanedione, and4-[(1H-imidazol-1-yl)phenylmethyl]-1,2-benzenediamine in place of4-(1H-imidazol-1-ylmethyl)-1,2-benzenediamine.

(c) 6-[(1H-imidazol-1-yl)(phenyl)methyl]-2,3-dimethylquinoxalinemonohydrate; mp. 82.9° C. (comp. 6) was prepared following substantiallythe same procedures as in example 17b except that 2,3-butanedione wasused in place of ethanedial.

EXAMPLE 18

(a) A mixture of 8.1 parts of4-[(3-fluorophenyl)(1H-imidazol-1-yl)methyl]-1,2-benzenediamine, 5 partsof ethanedial and 80 parts of methanol was stirred at refluxtemperature. Upon complete reaction, the mixture was evaporated to dryand the residue was taken up in water. The product was extracted withtrichloromethane. The extract was dried, filtered and evaporated. Theresidue was purified by column chromatography over silica gel using amixture of dichloromethane and methanol (98:2 by volume) was eluent. Thepure fractions were collected and the eluent was evaporated. The residuewas crystallized from 2,2'-oxybispropane. The product was filtered offand dried, yielding 6.35 parts (74.5%) of6-[(3-fluorophenyl)(1H-imidazol-1-yl)methyl]quinoxaline; mp. 109.7° C.(comp. 15).

(b)6-[(3-fluorophenyl)-(1H-imidazol-1-yl)methyl]-2,3-dimethylquinoxaline;mp. 81.4° C. (comp. 19) was prepared following substantially the sameprocedures as in example 18a except that 2,3-butanedione was used inplace of ethanedial.

EXAMPLE 19

To a cooled (0°-5° C.) solution of 4.5 parts of6-[1-(1H-imidazol-1-yl)-2-methylpropyl]quinoxaline in 79 parts ofmethanol were added portionwise 4.5 parts of sodium tetrahydroborate.After stirring for 3 hours at 0°-5° C., water was added. The product wasextracted with dichloromethane and the extract was dried, filtered andevaporated. The residue was purified by column chromatography (silicagel; CH₂ Cl₂ /CH₃ OH/NH₄ OH 90:10:0.1). The eluent of the desiredfraction was evaporated and the residue was converted into theethanedioate (2:5) salt in ethanol, yielding 1 part (11.5%) of1,2,3,4-tetrahydro-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]quinoxalineethanedioate(2:5) hemihydrate; mp. 145.6° C. (comp. 1).

EXAMPLE 20

(a) A mixture of 9.1 parts of4-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-1,2-benzenediamine, 3.7parts of ethyl 2-oxopropanoate and 160 parts of methanol was stirred for30 minutes at reflux temperature. The reaction mixture was evaporated todry. The residue was crystallized from a mixture of 36 parts of2-propanone and 4 parts of methanol. After stirring for 30 minutes atroom temperature, the precipitated product was filtered off (thefiltrate was set aside) and recrystallized from a mixture of methanoland dichloromethane. The product was filtered off and dried, yielding 3parts (28.1%) of6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-methyl-2(1H)-quinoxalinone;mp. 270.9° C. (comp. 73). The filtrate (see above) was evaporated andthe residue was purified by column chromatography over silica gel usinga mixture of dichloromethane and methanol (95:5 by volume) as eluent.The pure fractions were collected and the eluent was evaporated. Theresidue was taken up in a mixture of 2-butanone and 2-propanone and thewhole was allowed to stand for a few days. the precipitated product wasfiltered off and dried, yielding 1.4 parts (11.5%) of7-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-methyl-2(1H)-quinoxalinone hemihydrate; mp.201.9° C. (comp. 81).

(b)6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-(2-methylpropyl)-2(1H)-quinoxalinone;mp. 197.4° C. (comp. 101) and7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-(2-methylpropyl)-2(1H)-quinoxalinone;mp. 173.5° C. (comp. 102) were prepared following substantially the sameprocedures as in example 20a except that ethyl 4-methyl-2-oxopentanoatewas used in place of ethyl 2-oxopropanoate, and4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-1,2-benzenediamine in place of4-[(3-chloro-phenyl)-(1H-imidazol-1-yl)methyl]-1,2-benzenediamine.

(c)7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-(1-methylethyl)-2(1H)-quinoxalinone;mp. 186.7° C. (comp. 106) and6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-(1-methylethyl)-2(1H)-quinoxalinone;mp. 187.4° C. (comp. 117) were prepared following substantially the sameprocedures as in example 20b except that ethyl 3-methyl-2-oxobutanoatewas used in place of ethyl 4-methyl-2-oxopentanoate.

(d) 6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-phenyl-2(1H)quinoxalinone;mp. 209.6° C. (comp. 89) and7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-phenyl-2(1H)quinoxalinone; mp.281.0° C. (comp. 91) were prepared following substantially the sameprocedures as in example 20b except that methyl α-oxobenzenacetate wasused in place of ethyl 4-methyl-2-oxopentanoate.

(e) ethyl3,4-dihydro-7-(1H-imidazol-1ylmethyl)-α-methyl-3-oxo-2-quinoxalineacetate;mp. 208.1° C. (comp. 87) and ethyl3,4-dihydro-6-(1H-imidazol-1ylmethyl)-α-methyl-3-oxo-2-quinoxalineacetate;mp. 223.4° C. (comp. 88) were prepared following substantially the sameprocedures as in example 20a except that diethyl2-methyl-3-oxo-1,4-butanedioate was used in place of ethyl2-oxopropanoate, and 4-[(1H-imidazol-1-yl)methyl]-1,2-benzenediamine inplace of 4-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-1,2-benzenediamine.

EXAMPLE 21

(a) To a stirred and cooled (0° C.) solution of 9.1 parts of4-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-1,2-benzenediamine in 80parts of acetic acid and 20 parts of water were added portionwise 5.8parts of ethyl 4-methyl-2-oxopentanoate. Upon completion, stirring wascontinued for 4 hours at room temperature. The reaction mixture waspoured into 100 parts of water and the whole was neutralized with asodium hydroxide solution 3N. The product was extracted withdichloromethane. The extract was dried, filtered and evaporated. Theresidue was purified by column chromatography over silica gel using amixture of dichloromethane and methanol (95:5 by volume) as eluent. Thepure fractions were collected and the eluent was evaporated. The residuewas crystallized from a mixture of 2-propanone and 1,1'-oxybisethane.The product was filtered off (the filtrate was set aside) and dried,yielding 1.5 parts (12.6%) of 6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-(2-methylpropyl)-2(1H)-quinoxalinone; mp.209.7° C. (comp. 178)

The filtrate (see above) was evaporated and the residue was furtherpurified by column chromatography (HPLC) over silica gel using a mixtureof dichloromethane and methanol (95:5 by volume) as eluent. The purefractions were collected and the eluent was evaporated. The residue wascrystallized twice, yielding 1.2 parts (10.0%) of7-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-(2-methylpropyl)-2(1H)-quinoxalinone;mp. 215.2° C. (comp. 177).

(b)6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-(1-methylethyl)]-2(1H)-quinoxalinone;mp. 188.8° C. (comp. 181) and7-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-3-(1-methylethyl)]-2(1H)-quinoxalinone(comp. 313) were prepared following substantially the same procedures asin example 21a except that methyl 3-methyl-2-oxobutanoate was used inplace of ethyl 4-methyl-2-oxopentanoate.

EXAMPLE 22

A mixture of 8.8 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-1,2-benzenediamine, 3 parts of2-oxoacetic acid monohydrate and 80 parts of methanol was stirred for 4hours at reflux temperature. The reaction mixture was evaporated to dryand the residue was purified by column chromatography over silica gelusing a mixture of dichloromethane and methanol (98:2 by volume) aseluent. The fractions with the two isomers were collected and the eluentwas evaporated. The isomers were separated by crystallization; firstfrom a mixture of 2-butanone and 2-propanone and then from 2-butanone.

The first product was filtered off and dried, yielding 1.25 parts(12.3%) of 7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone;mp. 246.3° C. (comp. 90). The second product was filtered off and dried,yielding 0.5 parts (4.9%) of6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone; mp. 193.9°C. (comp. 94).

EXAMPLE 23

(a) To a stirred and cooled (0° C.) mixture of 9 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-1,2-benzenediamine in 80 partsof acetic acid and 20 parts of water were added 5 parts of2-oxopentanoic acid. The reaction mixture was stirred for 12 hours atroom temperature. The whole was poured into ice water and neutralizedwith a sodium hydroxide solution 3N. The product was extracted threetimes with 130 parts of dichloromethane. The combined extracts weredried, filtered and evaporated. For obtaining6-[1-(1H-imidazol-1yl)-2-methylpropyl]-3-propyl-2(1H)-quinoxalinone, theresidue was purified by column chromatography (HPLC) over silica gelusing a mixture of dichloromethane and methanol (98:2 by volume) aseluent. The pure fractions were collected and the eluent was evaporated.The residue was crystallized from a mixture of 2-butanone and1,1'-oxybisethane. The precipitated product was filtered off and stirredin cold 4-methyl-2-pentanone. After filtration, the product wasrecrystallized from a mixture of methanol and 2-propanone, yielding 1.6parts (13.2%) of the above product; mp. 259.7° C. (comp. 100).

For obtaining7-[1-(1H-imidazol-1yl)-2-methylpropyl]-3-propyl-2(1H)-quinoxalinone, theresidue was purified by column chromatography over silica gel using amixture of dichloromethane, 2-propanol and ammonium hydroxide (90:10:0.1by volume) as eluent. The pure fractions were collected and the eluentwas evaporated. The residue was crystallized from a mixture ofacetonitrile and 1,1'-oxybisethane. The precipitated product wasfiltered off and recrystallized first three times from a mixture ofmethanol and acetonitrile and then twice from a mixture of methanol,ethyl acetate and 2-propanol, yielding 1.45 parts (12.0%) of the aboveproduct; mp. 176.0° C. (comp. 140).

(b) 3-ethyl-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone;mp. 203.7° C. (comp. 104) and3-ethyl-7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone(comp. 314) were prepared following substantially the same procedures asin example 23a were used except that 2-oxobutanoic acid was used inplace of 2-oxopentanoic acid.

EXAMPLE 24

A solution of 15 parts of4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-1,2-benzenediamine and 12.5parts of diethyl 2-oxo-1,3-propanedioate in 80 parts of ethanol wasstirred for 2 hours at reflux temperature. The reaction mixture wasevaporated and the residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (95:5 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated. The residue was crystallized from a mixture of acetonitrileand ethanol. The product was filtered off and dried, yielding 3.1 parts(14.0%) of ethyl3,4-dihydro-7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-oxo-2-quinoxalinecarboxylate;mp. 229.7° C. (comp. 107).

The filtrate of the crystallization was evaporated and the residue wasrecrystallized from a mixture of acetonitrile and ethanol. The productwas filtered off and dried, yielding 2.2 parts (10.0%) of ethyl3,4-dihydro-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-oxo-2-quinoxalinecarboxylate;mp. 184.8° C. (comp. 116).

EXAMPLE 25

A mixture of 7 parts ofN-[4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrophenyl]-β-oxobenzenepropanamide,7.03 parts of potassium carbonate and 70 parts of water was stirred for1.5 hours at reflux temperature. After cooling, the whole was treatedwith a hydrochloric acid solution 3N to pH 7. The product was extractedwith dichloromethane (3×104 parts). The combined extracts were dried,filtered and evaporated. The residue was taken up in ethanol. Theproduct was filtered off and dried, yielding 5.1 parts (73.0%) of3-benzoyl-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone,N⁴ -oxide monohydrate; mp. 210.0° C. (comp. 154).

EXAMPLE 26

A mixture of 12 parts ofN-[4-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-2-nitrophenyl]-3-oxobutanamideand 120 parts of sodium hydroxide solution 6.5% was stirred for 15minutes at 80° C. After cooling, the product was obtained, yielding10.25 parts (100%) of6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-2(1H)-quinoxalinone, N⁴-oxide (comp. 175).

EXAMPLE 27

To a stirred mixture of 11 parts of4-chloro-N-[4-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2-nitrophenyl]benzeneacetamideand 49 parts of pyridine were added 3.6 parts of 2-methyl-2-propanol,potassium salt under a nitrogen atmosphere. After stirring for 1 hour atroom temperature, the reaction mixture was poured into ice-water. Thewhole was neutralized with HCl 3N and the product was extracted withdichloromethane. The extract was dried, filtered and evaporated. Theresidue was purified by column chromatography (silica gel; CH₂ Cl₂ /CH₃OH 98:2). The eluent of the desired fraction was evaporated, yielding6.6 parts (61.8%) of3-(4-chlorophenyl)-6-[2-methyl-1-(1H-1,2,4-triazol-1-yl)propyl]-2(1H)-quinoxalinone,N⁴ -oxide (comp. 221).

EXAMPLE 28

A solution of 14.5 parts of3-fluoro-N-[4-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-nitrophenyl]benzeneacetamidein 49 parts of pyridine and 10 parts of a potassium hydroxide solution20% was stirred for 1 hour at 85° C. The reaction mixture was pouredinto crushed ice and neutralized with a sulfuric acid solution 2N. Afterevaporation, the residue was purified by column chromatography oversilica gel using a mixture of dichloromethane, methanol and ammoniumhydroxide (95:5:0.5 by volume) as eluent. The pure fractions werecollected and the eluent was evaporated. The residue was crystallizedfrom a mixture of dichloromethane and 2,2'-oxybispropane. The productwas filtered off and dried, yielding 4.3 parts (67.6%) of3-(3-fluorophenyl)-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone,N⁴ -oxide; mp. 212.9° C. (comp. 166).

EXAMPLE 29

A mixture of 5 parts of 4-(1H-imidazol-1-ylmethyl)-1,2-benzenediamine, 4parts of diethyl ethanedioiate and 40 parts of methanol was stirred for4 hours at room temperature. The precipitated product was filtered offand dried, yielding 4 parts (62.3%) of6-(1H-imidazol-1-ylmethyl)-2,3(1H,4H)-quinoxalinedione; mp. >300° C.(comp. 315).

EXAMPLE 30

A solution of 3 parts of6-[1-(1H-imidazol-1-yl)pentyl]-3-phenyl-2(1H)-quinoxalinone, N⁴ -oxidein 80 parts of methanol was hydrogenated over night at 2.10⁵ Pa and atroom temperature with 0.5 parts of Raney nickel catalyst. The catalystwas filtered off over diatomaceous earth and the filtrate wasevaporated. The residue was crystallized from acetonitrile, yielding 2.5parts (87.2%) of6-[1-(1H-imidazol-1-yl)pentyl]-3-phenyl-2(1H)-quinoxalinone; mp. 192.4°C. (comp. 162).

EXAMPLE 31

A mixture of 10.25 parts of6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-2(1H)-quinoxalinone, N⁴-oxide, 120 parts of a sodium hydroxide solution 6.5% and 120 parts ofwater was hydrogenated for 1 hour in a Parr apparatus at 3.10⁵ Pa and atroom temperature with 10 parts of a Raney nickel catalyst under nitrogenatmosphere. The whole was filtered over diatomaceous earth and thefiltrate was treated with a hydrochloric acid solution 3N to pH 7. Theproduct was extracted with a mixture of dichloromethane and methanol.The extract was dried, filtered and evaporated. The residue was purifiedby column chromatography over silica gel using a mixture ofdichloromethane and methanol (96:4 by volume) as eluent. The purefractions were collected and the eluent was evaporated. The residue wascrystallized from a mixture of 2-butanone, 2-propanone and1,1'-oxybisethane. The product was filtered off and dried, yielding 0.95parts (9.7%) of 6-[(3-chlorophenyl)(1H-imidazol-1-yl)methyl]-2(1H)-quinoxalinone; mp. 253.0° C. (comp. 176).

EXAMPLE 32

A mixture of 3.9 parts of3-(3,4-dimethoxyphenyl)-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone,N⁴ -oxide, 3.3 parts of sodium dithionate, 55.3 parts of ethanol and 30parts of water was refluxed for 1/2 hour. After cooling, the reactionmixture was partitioned between water and dichloromethane. The organiclayer was dried, filtered and evaporated. The residue was purified bycolumn chromatography (silica gel; CH₂ Cl₂ /CH₃ OH 95:5). The eluent ofthe desired fraction was evaporated and the residue was crystallizedfrom methanol and ethyl acetate, yielding 2.1 parts (56.4%) of3-(3,4-dimethoxyphenyl)-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone;mp. 242.6° C. (comp. 236).

EXAMPLE 33

A mixture of 3 parts of7-(1H-imidazol-1-ylmethyl)-3-methyl-2(1H)-quinoxalinone, 0.3 parts of asodium hydroxide dispersion 50% and 28 parts of N,N-dimethylformamidewas stirred for 1.5 hours at room temperature. 2 Parts of iodomethanewere added and stirring was continued for 12 hours at room temperatureunder nitrogen atmosphere. The reaction mixture was evaporated to dryand the residue was taken up in water and potassium carbonate. Theproduct was extracted with dichloromethane. The extract was dried,filtered and evaporated. The residue was purified by columnchromatography over silica gel using a mixture of dichloromethane andmethanol (98:2 by volume) as eluent. The pure fractions were collectedand the eluent was evaporated. The residue was crystallized from amixture of 2,2'-oxybispropane and 2-propanone. The product was filteredoff and dried, yielding 2.2 parts (66.8%) of7-(1H-imidazol-1-ylmethyl)-1,3-dimethyl-2(1H)-quinoxalinone hemihydrate;mp. 128.6° C. (comp. 79).

EXAMPLE 34

A mixture of 5 parts of6-[(1H-imidazol-1-yl)phenylmethyl]-3-methyl-2(1H)-quinoxalinone, 3.3parts of sodium hydroxide and 30 parts of water was stirred for 1 hourat room temperature. 5 Parts of hydroxylamine-O-sulfonic acid were addedand the reaction mixture was stirred for 4 hours at 20° C. The productwas extracted with dichloromethane (3×65 parts). The combined extractswere dried, filtered and evaporated. The residue was purified by columnchromatography over silica gel using a mixture of dichloromethane andmethanol (95:5 by volume) as eluent. The pure fractions were collectedand the eluent was evaporated. The residue was crystallized from amixture of 2-propanone and 1,1'-oxybisethane. The product was filteredoff and dried, yielding 2 parts (38.2%) of1-amino-6-[(1H-imidazol-1-yl)phenylmethyl]-3-methyl-2(1H)-quinoxalinone;mp. 192.8° C. (comp. 85).

EXAMPLE 35

A solution of 4.25 parts of ethyl3,4-dihydro-7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-oxo-2-quinoxalinecarboxylatein 20 parts of a sodium hydroxide solution 1N was stirred for 4 hours atroom temperature. The reaction mixture was treated with a dilutedsulfuric acid solution to pH 5.5. After concentration, the residue wascrystallized from pyridine. The product was filtered off and dried,yielding 1 part (24.6%) of3,4-dihydro-7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-oxo-2-quinoxalinecarboxylicacid; mp. 237.5° C. (comp. 129).

EXAMPLE 36

A mixture of 6 parts of7-(1H-imidazol-1-ylmethyl)-3-methyl-2(1H)-quinoxalinone and 40 parts ofphosphoryl chloride was stirred for 2 hours at reflux temperature. Thereaction mixture was evaporated to dry. The residue was taken up in 300parts of ice water and the whole was neutralized with potassiumcarbonate. The product was extracted three times with 65 parts ofdichloromethane. The combined extracts were dried, filtered andevaporated. The residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (98:2 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated. The residue was crystallized from 1,1'-oxybisethane. Theproduct was filtered off and dried, yielding 1.6 parts (59.4%) of3-chloro-6-(1H-imidazol-1-ylmethyl)-2-methylquinoxaline; mp. 115.8° C.(comp. 22).

EXAMPLE 37

A solution of 0.3 parts of sodium in 24 parts of 1-propanol was added to2.4 parts of 3-chloro-6-(1H-imidazol-1-ylmethyl)-2-methylquinoxaline.The whole was stirred for 2 hours at reflux temperature. The reactionmixture was evaporated and the residue was extracted three times with 65parts of dichloromethane. The combined extracts were dried, filtered andevaporated. The residue was purified by column chromatography oversilica gel using a mixture of dichloromethane and methanol (98:2 byvolume) as eluent. The pure fractions were collected and the eluent wasevaporated. The residue was crystallized from a mixture of pentane and2,2'-oxybispropane. The product was filtered off and dried, yielding 1.5parts (57.1%) of6-(1H-imidazol-1-ylmethyl)-2-methyl-3-propoxyquinoxaline; mp. 85.5° C.(comp. 24).

EXAMPLE 38

A mixture of 5.5 parts of3-chloro-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2-methylquinoxaline, 9parts of an aqueous N-methylmethanamine solution 40% and 48 parts ofmethanol was stirred for 12 hours at 140° C. After cooling, the reactionmixture was evaporated to dry and the residue was purified by columnchromatography over silica gel using a mixture of dichloromethane andmethanol (98:2 by volume) as eluent. The pure fractions were collectedand the eluent was evaporated. The residue was crystallized frompetroleum ether. The product was filtered off and dried, yielding 0.9parts (15.9%) of7-[1-(1H-imidazol-1-yl)-2-methylpropyl]-N,N,3-trimethyl-2-quinoxazolinamine;mp. 116.7° C. (comp. 47).

All other compounds listed in tables 7-11 were obtained by analogousmethods of preparation as described in examples 14-38, the actual methodof preparation being indicated in column 2 (Ex. No.)

                                      TABLE 7                                     __________________________________________________________________________     ##STR33##                                                                    Comp. No.                                                                           Ex. No.                                                                            R  X.sup.1X.sup.2                                                                        Y      mp.(°C.)/base/salt                        __________________________________________________________________________    1     19   H  CHCH    i-C.sub.3 H.sub.7                                                                    145.6° C./0.5H.sub.2 O/                                                2.5(COOH).sub.2                                  2     --   H  CHCH    H      --                                               3     --   H  CHCH    C.sub.6 H.sub.5                                                                      --                                               4     --   H  CHCH    4ClC.sub.6 H.sub.4                                                                   --                                               __________________________________________________________________________

                                      TABLE 8                                     __________________________________________________________________________     ##STR34##                                                                    Comp.                                                                             Ex.                                    mp. (°C.)/                  No. No.                                                                              R   X.sup.1X.sup.2                                                                        Y         p R.sup.1                                                                              R.sup.2                                                                            base/salt                          __________________________________________________________________________     5  17 H   CHCH    C.sub.6 H.sub.5                                                                         6 H      H    126.8                               6  17 H   CHCH    C.sub.6 H.sub.5                                                                         6 CH.sub.3                                                                             CH.sub.3                                                                           82.9/H.sub.2 O                      7  17 H   CHCH    CH.sub.3  6 H      H    135.1                               8  17 H   CHCH    H         6 C.sub.6 H.sub.5                                                                      C.sub.6 H.sub.5                                                                    159.3                               9  18 H   CHCH    i-C.sub.3 H.sub.7                                                                       6 H      H    128.6                              10  18 H   CHCH    H         6 H      H    144.1                              11  18 H   CHCH    4FC.sub.6 H.sub.4                                                                       6 H      H    131.6                              12  18 H   CHCH    C.sub.2 H.sub.5                                                                         6 H      H    72.4                               13  18 H   CHCH    2-thienyl 6 H      H    93.7                               14  18 H   CHCH    4FC.sub.6 H.sub.4                                                                       6 CH.sub.3                                                                             CH.sub.3                                                                           82.0/H.sub.2 O                     15  18 H   CHCH    3FC.sub.6 H.sub.4                                                                       6 H      H    109.7                              16  18 H   CHCH    2FC.sub.6 H.sub.4                                                                       6 H      H    79.7                               17  18 H   CHCH    CH.sub.3  6 CH.sub.3                                                                             CH.sub.3                                                                           76.2/0.5H.sub.2 O                  18  18 H   CHCH    3ClC.sub.6 H.sub.4                                                                      6 H      H    70.3/0.5H.sub.2 O                  19  18 H   CHCH    3FC.sub.6 H.sub.4                                                                       6 CH.sub.3                                                                             CH.sub.3                                                                           81.4                               20  36 H   CHCH    C.sub.6 H.sub.5                                                                         6 Cl     CH.sub.3                                                                           164.7                              21  37 H   CHCH    C.sub.6 H.sub.5                                                                         6 CH.sub.3 O                                                                           CH.sub.3                                                                           117.7                              22  36 H   CHCH    H         7 Cl     CH.sub.3                                                                           115.8                              23  37 H   CHCH    H         7 CH.sub.3 O                                                                           CH.sub.3                                                                           151.8                              24  37 H   CHCH    H         7 C.sub.3 H.sub.7 O                                                                    CH.sub.3                                                                           85.5                               25  37 H   CHCH    H         7 i-C.sub.3 H.sub.7 O                                                                  CH.sub.3                                                                           109.6                              26  36 H   CHCH    H         6 Cl     CH.sub.3                                                                           --                                 27  37 H   CHCH    H         6 C.sub.3 H.sub.7 O                                                                    CH.sub.3                                                                           132.0                              28  37 H   CHCH    H         6 i-C.sub.3 H.sub.7 O                                                                  CH.sub.3                                                                           117.5                              29  37 H   CH CH   H         7 1 .sub.--H-imidazolyl                                                                CH.sub.3                                                                           164.1                              30  18 H   CHCH    2FC.sub.6 H.sub.4                                                                       6 CH.sub.3                                                                             CH.sub.3                                                                           94.5                               31  37 H   CHCH    H         6 CH.sub.3 O                                                                           CH.sub.3                                                                           150.7                              32  37 H   CHCH    C.sub.6 H.sub.5                                                                         6 C.sub.3 H.sub.7 O                                                                    CH.sub.3                                                                           125.6                              33  36 H   CHCH    H         7 Cl     H    --                                 34  37 H   CHCH    H         7 CH.sub.3 O                                                                           H    121.0                              35  18 H   CHCH    2ClC.sub.6 H.sub.4                                                                      6 H      H    114.7                              36  36 H   CHCH    C.sub.6 H.sub.5                                                                         7 Cl     CH.sub.3                                                                           --                                 37  37 H   CHCH    C.sub.6 H.sub.5                                                                         7 CH.sub.3 O                                                                           CH.sub.3                                                                           131.2                              38  36 H   CHCH    H         6 Cl     H    --                                 39  37 H    CHCH   H         6 CH.sub.3 O                                                                           H    123.2                              40  18 H   CHCH    4CH.sub.3C.sub.6 H.sub.4                                                                6 H      H    132.9                              41  14 H   CHCH    H         5 H      H    121.2                              42  18 H   CHCH    3CF.sub.3C.sub.6 H.sub.4                                                                6 H      H    96.5/H.sub.2 O                     43  36 H   CHCH    i-C.sub.3 H.sub.7                                                                       6 Cl     CH.sub.3                                                                           --                                 44  38 H   CHCH    i-C.sub.3 H.sub.7                                                                       6 NH.sub.2                                                                             CH.sub.3                                                                           238.2                              45  36 H   CHCH    i-C.sub.3 H.sub.7                                                                       7 Cl     CH.sub.3                                                                           --                                 46  37 H   CHCH    i-C.sub.3 H.sub.7                                                                       7 CH.sub.3 O                                                                           CH.sub.3                                                                           124.6                              47  38 H   CHCH    i-C.sub.3 H.sub.7                                                                       7 (CH.sub.3).sub.2 N                                                                   CH.sub.3                                                                           116.7                              48  15 CH.sub.3                                                                          CHCH    C.sub.6 H.sub.5                                                                         6 H      H    134.5                              49  15 H   CHN     C.sub.6 H.sub.5                                                                         6 H      H    96.0                               50  15 H   NCH     C.sub.6 H.sub.5                                                                         6 H      H    98.1                               51  16 H   CHCH    1 .sub.--H-imidazol-1-yl                                                                6 H      H    240.3/2*                           52  -- H   CHN     C.sub.6 H.sub.5                                                                         6 CH.sub.3                                                                             CH.sub.3                                                                           --                                 53  -- H   CHN     i-C.sub.3 H.sub.7                                                                       6 CH.sub.3                                                                             CH.sub.3                                                                           --                                 54  -- CH.sub.3                                                                          CHCH    i-C.sub.3 H.sub.7                                                                       6 H      H    --                                 55  -- H   CHCH    CH.sub.3CC                                                                              6 H      H    --                                 56  -- H   CHCH    CH.sub.3CHCH                                                                            6 H      H    --                                 __________________________________________________________________________     *= 4-methylbenzenesulfonate                                              

In the previous and following tables p indicates the position of the1H-azol-1-yl-methyl moiety on the quinoxaline ring.

    TABLE 9      ##STR35##       Comp. Ex.        mp.(°C.)/ No. No. R X.sup.1X.sup.2 Y p R.sup.3     R.sup.4 n base/salt        57 20 H CHCH C.sub.6 H.sub.5 6 H CH.sub.3 0 254.0  58 20 H CHCH H 7 H     CH.sub.3 0 297.6  59 20 H CHCH H 6 H CH.sub.3 0 271.3  60 20 H CHCH     C.sub.6 H.sub. 5 7 H CH.sub.3 0 218.4  61 22 H CHCH H 7 H H 0 253.6  62     22 H CHCH H 6 H H 0 272.9  63 34 H CHCH H 7 NH.sub.2 CH.sub.3 0 178.8     64 20 H CHCH 3-CF.sub.3C.sub.6 H.sub.4 6 H CH.sub.3 0 >300(dec.)  65 20     H CHCH CH.sub.3 6 H CH.sub.3 0 268.2  66 20 H CHCH H 7 H C.sub.6 H.sub.5     0 293.8  67 20 H CHCH H 6 H C.sub.6 H.sub.5 0 203.1  68 20 H CHCH     2FC.sub.6 H.sub.4 6 H CH.sub.3 0 273.5  69 20 H CHCH 3FC.sub.6 H.sub.4 6     H CH.sub.3 0 275.0   70 20 H CHCH 4FC.sub.6 H.sub.4 6 H CH.sub.3 0 271.7      71 20 H CHCH i-C.sub.3 H.sub.7 7 H CH.sub.3 0 249.8  72 33 H CHCH H 6     CH.sub.3 CH.sub.3 0 191.0  73 20 H CHCH 3ClC.sub.6 H.sub.4 6 H CH.sub.3     0 270.9  74 33 H CHCH H 7 C.sub.4 H.sub.9 CH.sub.3 0 116.0  75 33 H CHCH     C.sub.6 H.sub.5 6 C.sub.4 H.sub.9 CH.sub.3 0 139.9  76 33 H CHCH C.sub.6     H.sub.5 6 CH.sub.3 CH.sub.3 0 214.5  77 20 H CHCH i-C.sub.3 H.sub.7 6 H     CH.sub.3 0 192.5  78 20 H CHCH C.sub.3 H.sub.7 6 H CH.sub.3 0 234.0  79     33 H CHCH H 7 CH.sub.3 CH.sub.3 0 128.6/0.5H.sub.2 O  80 33 H CHCH H 6     C.sub.4 H.sub.9 CH.sub.3 0 128.4  81 20 H CHCH 3ClC.sub.6 H.sub.4 7 H     CH.sub.3 0 201.9/0.5H.sub.2 O  82 20 H CHCH CH.sub.3 7 H CH.sub.3 0     228.8  83 20 H CHCH 3FC.sub.6 H.sub.4 7 H CH.sub.3 0 209.9  84 20 H CHCH     4FC.sub.6 H.sub.4 7 H CH.sub.3 0 177.3/0.5H.sub.2      O          (COOH).sub.2  85 34 H CHCH C.sub.6 H.sub.5 6 NH.sub.2     CH.sub.3 0 192.8  86 22 H CHCH C.sub.6 H.sub.5 6 H H 0 225.0  87 20 H     CH CH H 6 H * 0 208.1  88 20 H CHCH H 7 H * 0 223.4  89 20 H CHCH     i-C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 0 209.6  90 22 H CHCH i-C.sub.3     H.sub.7 7 H H 0 246.3  91 20 H CHCH i-C.sub.3 H.sub.7 7 H C.sub.6     H.sub.5 0 281.0  92 34 H CHCH H 6 NH.sub.2 CH.sub.3 0 220.7  93 20 H     CHCH 3-pyridinyl 6 H CH.sub.3 0 237.4/0.5H.sub.2 O  94 22 H CHCH     i-C.sub.3 H.sub.7 6 H H 0 198.2  95 22 H CHCH C.sub.6 H.sub.5 7 H H 0     187.6  96 20 H CHCH C.sub.6 H.sub.5 6 H C.sub.6 H.sub.5 0 251.0  97 20 H C     HCH c.C.sub.3 H.sub.5 6 H CH.sub.3 0 275.1  98 20 H CHCH i-C.sub.4     H.sub.9 6 H CH.sub.3 0 205.4  99 20 H CHCH C.C.sub.3 H.sub.5 7 H     CH.sub.3 0 203.0 100 23 H CHCH i-C.sub.3 H.sub.7 6 H C.sub.3 H.sub.7 0     259.7 101 20 H CHCH i-C.sub.3 H.sub.7 6 H i-C.sub.4 H.sub.9 0 197.4 102     20 H CHCH i-C.sub.3 H.sub.7 7 H i-C.sub.4 H.sub.9 0 173.5 103 20 H CHCH     C.sub.2 H.sub.5 6 H CH.sub.3 0 211.9 104 23 H CHCH i-C.sub.3 H.sub.7 6 H     C.sub.2 H.sub.5 0 203.7 105 20 H CHCH i-C.sub.4 H.sub.9 7 H CH.sub.3 0     189.0 106 20 H CHCH i-C.sub.3 H.sub.7 7 H i-C.sub.3 H.sub.7 0 186.7 107     24 H CHCH i-C.sub.3 H.sub.7 6 H COOC.sub.2 H.sub.5 0 229.7 108 20 H CHCH     i-C.sub.3 H.sub.7 6 CH.sub.3 CH.sub.3 0 132.6/0.5H.sub.2 O 109 20 H CHCH     C.sub.2 H.sub.5 7 H CH.sub.3 0 197.4 110 20 H CHCH C.sub.4 H.sub.9 6 H     CH.sub.3 0 201.6 111 20 H CHCH 4-ClC.sub.6 H.sub.4 6 H CH.sub.3 0 228.7     112 20 H CHCH 4-ClC.sub.6 H.sub.4 7 H CH.sub.3 0 160.8 113 20 H CHCH     4-CH.sub.3 OC.sub.6 H.sub.4 6 H CH.sub.3 0 199.7 114 23 H CHCH C.sub.6     H.sub.5 6 H C.sub.2 H.sub. 5 0 280.0 115 23 H CHCH C.sub.6 H.sub.5 7 H     C.sub.2 H.sub.5 0 211.2 116 24 H CHCH i-C.sub.3 H.sub.7 7 H COOC.sub.2     H.sub.5 0 184.8 117 20 H CHCH i-C.sub.3 H.sub.7 6 H i-C.sub.3 H.sub.7 0     187.4 118 22 H CHCH i-C.sub.4 H.sub.9 7 H H 0 203.6 119 20 H CHCH     4-CH.sub.3C.sub.6      H.sub.4 7 H CH.sub.3 0 150.4 120 20 H CHCH 4-CH.sub.3C.sub.6 H.sub.4 6     H CH.sub.3 0 222.5 121 20 H CHCH C.sub.4 H.sub.9 7 H CH.sub.3 0 143.1     122 22 H CHCH 2-thienyl 7 H H 0 223.1 123 20 H CHCH 2-thienyl 6 H     CH.sub.3 0 254.5 124 20 H CHCH  4-CH.sub.3 OC.sub.6 H.sub.4 7 H CH.sub.3     0 203.5 125 20 H CHN i-C.sub.3 H.sub.7 7 H CH.sub.3 0 204.8 126 20 H     CHCH c.C.sub.6 H.sub.11 6 H CH.sub.3 0 174.8 127 20 H CHCH c.C.sub.3     H.sub.5 6 H C.sub.6 H.sub.5 0 >300 128 20 H CHCH 2-thienyl 7 H CH.sub.3     0 229.6 129 35 H CHCH i-C.sub.3 H.sub.7 6 H HOOC 0 237.5 130 22 H CHCH     c.C.sub.3 H.sub.5 7 H H 0 234.6 131 22 H CHCH C.sub.3 H.sub.7 7 H H 0     168.5 132 20 H CHCH CH.sub.3 6 H C.sub.6 H.sub.5 0 >300 133 22 H CHCH     c.C.sub.3 H.sub.5 6 H H 0 224.2 134 20 H CHCH c.C.sub.5 H.sub.9 6 H     C.sub.6 H.sub.5 0 127.9 135 20 H CHCH c.C.sub.6 H.sub.11 6 H C.sub.6     H.sub.5 0 193.4 136 22 H CHCH CH.sub.3 7 H H 0 252.4 137 20 H CHCH     C.sub.2 H.sub.5 6 H C.sub.6 H.sub.5 0 >300 138 22 H CHCH C.sub.3 H.sub.7     6 H H 0 161.9 139 22 H CHCH c.C.sub.6 H.sub.11 7 H H 0 278.9 140 23 H     CHCH i-C.sub.3 H.sub.7 7 H C.sub.3 H.sub.7 0 176.0 141 20 H CHCH     i-C.sub.4 H.sub.9 6 H C.sub.6 H.sub.5 0 245.0 142 22 H CHCH C.sub.2     H.sub.5 7 H H 0 201.3 143 22 H  CHCH c.C.sub.5 H.sub.9 7 H H 0 >300 144     22 H CHCH C.sub.4 H.sub.9 7 H H 0 170.0 145 20 H CHCH C.sub.4 H.sub.9 7     H C.sub.6 H.sub.5 0 198.4 146 20 H CHCH c.C.sub.5 H.sub.9 6 H CH.sub.3 0     221.1 147 20 H CHCH C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 0 249.4 148 22 H C     HN i-C.sub.3 H.sub.7 6 H H 0 209.2 149 20 H CHN i-C.sub.3 H.sub.7 6 H     CH.sub.3 0 211.0 150 20 H CHCH C.sub.2 H.sub.5 7 H C.sub.6 H.sub.5 0     272.2 151 26 H CHCH i-C.sub.3 H.sub.7 6 H H 1 208.7 152 25 H CHCH     CH.sub.3 6 H  C.sub.6 H.sub.5CO 1 260.4 153 25 H CHCH H 6 H C.sub.6     H.sub.5CO 1 250.4 154 25 H CHCH i-C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5CO     1 210.0/H.sub.2 O 155 28 H CHCH i-C.sub.3 H.sub.7 6 H 4ClC.sub.6 H.sub.4     1 181.3 156 28 H CHCH i-C.sub.3 H.sub.7 6 H 4FC.sub.6 H.sub.4 1 205.3     157 30 H CHCH i-C.sub.3 H.sub.7 6 H 4FC.sub.6 H.sub.4 0 240.8 158 28 H   N     CH i-C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 1 -- 159 30 H CHN i-C.sub.3     H.sub.7 6 H C.sub.6 H.sub.5 0 249.4 160 30 H aCHCH i-C.sub.3 H.sub.7 6 H     4ClC.sub.6 H.sub.4 0 236.6 161 28 H CHCH C.sub.4 H.sub.9 6 H C.sub.6     H.sub.5 1 240.5 162 30 H CHCH C.sub.4 H.sub.9 6 H C.sub.6 H.sub.5 0     192.4 163 30 H CHCH i-C.sub.3 H.sub.7 6 H C.sub.6      H.sub.5CO 0 161.5/0.5H.sub.2 O 164 28 H CHCH i-C.sub.3 H.sub.7 6 H     3ClC.sub.6 H.sub.4 1 177.4 165 30 H CHCH i-C.sub.3      H.sub.7 6 H 3ClC.sub.6 H.sub.4 0 179.7/H.sub.2      O 166 28 H CHCH i-C.sub.3 H.sub.7 6 H 3FC.sub.6 H.sub.4 1 212.9 167 30     H CHCH i-C.sub.3 H.sub.7 6 H 3FC.sub.6 H.sub.4 0 255.6 168 30 H CHCH H 6     H C.sub.6 H.sub.5CO 0 268.9 169 30 H CHCH CH.sub.3  6 H C.sub.6      H.sub.5CO 0 192.8 170 28 H CHCH 4ClC.sub.6 H.sub.4 6 H C.sub.6 H.sub.5     1 186.2 171 26 H CHCH H 6 H H 1 -- 172 30 H CHCH 4ClC.sub.6 H.sub.4 6 H     C.sub.6 H.sub.5 0 166.3/0.5H.sub.2 O 173 20 H CHCH 4-iC.sub.3      H.sub.7C.sub.6 H.sub.4 6 H CH.sub.3 0 237.6 174 20 H CHCH 4-iC.sub.3     H.sub.7C.sub.6 H.sub.4 7 H CH.sub.3 0 210.4 175 26 H CHCH 3ClC.sub.6     H.sub.4 6 H H 1 -- 176 31 H CHCH 3ClC.sub.6 H.sub.4 6 H H 0 253.0 177 21     H CHCH 3ClC.sub.6 H.sub.4 7 H i-C.sub.4 H.sub.9 0 215.2 178 21 H  CHCH     3ClC.sub.6 H.sub.4 6 H i-C.sub.4      H.sub.9 0 209.7 179 23 H CHCH 3ClC.sub.6 H.sub.4 7 H C.sub.3 H.sub.7 0     187.5 180 23 H CHCH 3ClC.sub.6 H.sub.4 6 H C.sub.3 H.sub.7 0 204.1 181     21 H CHCH 3ClC.sub.6 H.sub.4 6 H i-C.sub.3 H.sub.7 0 188.8 182 26 H CHCH     c.C.sub.6 H.sub.11 6 H H 1 -- 183 31 H CHCH c.C.sub.6 H.sub.11 6 H H 0     275.7 184 26 H CHCH 4-ClC.sub.6 H.sub.4 6 H H 1 -- 185 31 H CHCH     4-ClC.sub.6 H.sub.4 6 H H 0 149.2 186 26 H CHCH c.C.sub.5 H.sub.9 6 H H     1 -- 187 31 H CHCH c.C.sub.5 H.sub.9 6 H H 0 229.5 188 28 H CHCH     3-ClC.sub.6 H.sub.4 6 H C.sub.6 H.sub.5 1 236.1 189 26 H CHCH CH.sub.3 6     H H 1 -- 190 31 H CHCH CH.sub.3 6 H H 0 263.8 191 23 H CHCH 4ClC.sub.6     H.sub.4 6 H C.sub.3 H.sub.7 0 216.5 192 23 H CHCH 4ClC.sub.6 H.sub.4 7 H     C.sub.3 H.sub.7 0 222.0 193 21 H CHCH 4ClC.sub.6      H.sub.4 6 H CH.sub.2CH(CH.sub.3).sub.2 0 200.3 194 21 H CHCH 4ClC.sub.6     H.sub.4 7 H CH.sub.2CH(CH.sub.3).sub.2 0 203.9 195 21 H CHCH 4ClC.sub.6     H.sub.4 6 H i-C.sub.3 H.sub.7  0 200.9 196 30 H CHCH 3ClC.sub.6 H.sub.4     6 H C.sub.6 H.sub.5 0 245.5 197 26 H CHCH C.sub.2 H.sub.5 6 H H 1 -- 198     31 H CHCH C.sub.2 H.sub.5 6 H H 0 200.6 199 25 H CHCH C.sub.6 H.sub.5 6     H C.sub.6 H.sub.5CO 1 186.5/0.5H.sub.2 O 200 26 H CHCH 4FC.sub.6 H.sub.4     6 H H 1 -- 201 31 H CHCH 4FC.sub.6 H.sub.4 6 H H 0 259.5 202 26 H CHCH   C     2F.sub.6 H.sub.4 6 H H 1 -- 203 31 H CHCH 2FC.sub.6 H.sub.4 6 H H 0     220.3 204 26 H CHCH 3FC.sub.6 H.sub.4 6 H H 1 -- 205 31 H CHCH 3FC.sub.6     H.sub.4 6 H H 0 135.4/0.5H.sub.2 O 206 34 H CHCH i-C.sub.3 H.sub.7 6     NH.sub.2 CH.sub.3 0 -- 207 28 H CHN C.sub.6 H.sub.5 6 H C.sub.6 H.sub.5     1 -- 208 30 H CHN C.sub.6 H.sub.5 6 H C.sub.6 H.sub.5 0 259.3 209 28 H   C     CHH i-C.sub.3 H.sub.7 6 H 2CH.sub.3C.sub.6 H.sub.4 1 -- 210 30 H CHCH     i-C.sub.3 H.sub.7 6 H 2CH.sub.3C.sub.6 H.sub.4 0 154.2/0.5H.sub.2 O 211     27 H CHCH i-C.sub.3 H.sub.7 6 H 3-OCH.sub.3C.sub.6 H.sub.4 1 -- 212 30 H C     HCH i-C.sub.3 H.sub.7 6 H 3-OCH.sub.3C.sub.6 H.sub.4 0 225.0 213 27 H     CHCH i-C.sub.3 H.sub.7 6 H 2FC.sub.6      H.sub.4 1 -- 214 30 H CHCH i-C.sub.3 H.sub.7 6 H 2FC.sub.6 H.sub.4 0     230.1 215 27 H CHN i-C.sub.3 H.sub.7 6 H 4FC.sub.6 H.sub.4 1 -- 216 30 H C     HN i-C.sub.3 H.sub.7 6 H 4FC.sub.6 H.sub.4 0 268.0 217 28 H CHCH     i-C.sub.3 H.sub.7 6 H 4-CH.sub.3C.sub.6 H.sub.4 1 -- 218 30 H CHCH     i-C.sub.3 H.sub.7 6 H 4-CH.sub.3C.sub.6 H.sub.4 0 221.9 219 27 H CHN     i-C.sub.3 H.sub.7 6 H 3FC.sub.6 H.sub.4 1 -- 220 30 H CHN i-C.sub.3     H.sub.7 6 H 3FC.sub.6 H.sub.4 0 202.3 221 27 H  CHN i-C.sub.3 H.sub.7 6     H 4ClC.sub.6 H.sub.4 1 -- 222 30 H CHN i-C.sub.3 H.sub.7 6 H 4ClC.sub.6     H.sub.4 0 274.6 223 27 CH.sub.3 CHCH i-C.sub.3 H.sub.7 6 H C.sub.6     H.sub.5 1 -- 224 30 CH.sub.3 CHCH i-C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5     0 252.5 225 27 H CHCH i-C.sub.3 H.sub.7 6 H 4-BrC.sub.6 H.sub.4 1 -- 226     30 H CHCH i-C.sub.3 H.sub.7 6 H 4-BrC.sub.6 H.sub.4 0 226.0 227 27 H     CHCH i-C.sub.3 H.sub.7 6 H 3,4-F.sub.2C.sub.6 H.sub.3 1 -- 228 30 H CHCH     i-C.sub.3 H.sub.7 6 H 3,4-F.sub.2C.sub.6 H.sub.3 0 218.0 229 27 H  CHCH     i-C.sub.3 H.sub.7 6 H 3CH.sub.3C.sub.6 H.sub.4 1 -- 230 30 H CHCH     i-C.sub.3 H.sub.7 6 H 3CH.sub.3C.sub.6 H.sub.4 0 230.4 231 27 H CHCH     i-C.sub.3 H.sub.7 6 H 4-OCH.sub.3C.sub.6 H.sub.4 1 -- 232 30 H CHCH     i-C.sub.3 H.sub.7 6 H 4-OCH.sub.3C.sub.6 H.sub.4 0 157.8/H.sub.2 O 233     27 H CHCH 3,4Cl.sub.2C.sub.6 H.sub.3 6 H C.sub.6 H.sub.5 1 -- 234 30 H   C     CHH 3,4Cl.sub.2C.sub.6 H.sub.3 6 H C.sub.6 H.sub.5 0 262.5 235 27 H CHCH     i-C.sub.3 H.sub.7 6 H 3,4(OCH.sub.3).sub.2C.sub.6 H.sub.3 1 -- 236 32 H     CHCH i-C.sub.3 H.sub.7 6 H 3,4(OCH.sub.3).sub.2C.sub.6 H.sub.3 0 242.6     237 27 H CHCH i-C.sub.3 H.sub.7 6 H 2,4Cl.sub.2C.sub.6 H.sub.3 1 -- 238     32 H CHCH i-C.sub.3 H.sub.7 6 H 2,4Cl.sub.2C.sub.6 H.sub.3 0 178.0 239     27 H CHCH i-C.sub.3 H.sub.7 6 H 2-naphthalenyl- 1 -- 240 32 H CHCH     i-C.sub.3 H.sub.7 6 H 2-naphthalenyl- 0 274.9 241 27 H CHCH i-C.sub.3     H.sub.7 6 H 3,4,5(OCH.sub.3).sub.3C.sub.6 H.sub.2 1 -- 242 32 H CHCH     i-C.sub.3 H.sub.7 6 H 3,4,5(OCH.sub.3).sub.3C.sub.6 H.sub.2 0 266.6 243     27 H CHN i-C.sub.3 H.sub.7 6 H 3-thienyl- 1 -- 244 32 H CHN i-C.sub.3     H.sub.7 6 H 3-thienyl- 0 269.9 245 27 H CHCH i-C.sub.3 H.sub. 7 6 H     2-thienyl- 1 -- 246 32 H CHCH i-C.sub.3 H.sub.7 6 H 2-thienyl- 0 276.0     247 27 H CHCH i-C.sub.3 H.sub.7 6 H 2-OCH.sub.3C.sub.6 H.sub.4 1 -- 248     32 H CHCH i-C.sub.3 H.sub.7 6 H 2-OCH.sub.3C.sub.6 H.sub.4 0 169.8 249     27 H CHCH i-C.sub.3 H.sub.7 6 H 1-naphthalenyl- 1 -- 250 32 H CHCH     i-C.sub.3 H.sub.7 6 H 1-naphthalenyl- 0 183.5 251 27 H CHCH i-C.sub.3     H.sub.7 6 H 4-OC.sub.2 H.sub.5C.sub.6 H.sub.4 1 -- 252 32 H CHCH     i-C.sub.3 H.sub.7 6 H 4-OC.sub.2 H.sub.5C.sub.6 H.sub.4 0 129.5 253 27 H C     HCH i-C.sub.3 H.sub.7 6 H 2-ClC.sub.6 H.sub.4 1 -- 254 32 H CHCH     i-C.sub.3 H.sub.7 6 H 2-ClC.sub.6      H.sub.4 0 172.5 255 27 H CHCH i-C.sub.3 H.sub.7 6 H 3-OHC.sub.6 H.sub.4     1 -- 256 32 H CHCH i-C.sub.3 H.sub.7 6 H 3-OHC.sub.6 H.sub.4 0 252.6 257     27 H CHCH i-C.sub.3 H.sub.7 6 H 3-BrC.sub.6 H.sub.4 1 -- 258 32 H CHCH     i-C.sub.3 H.sub.7 6 H 3-BrC.sub.6 H.sub.4 0 157.8/0.5H.sub.2 O 259 20 H     CHN i-C.sub.3 H.sub.7 7 H 3-pyridinyl- 0 298.7 260 23 H CHN i-C.sub.3     H.sub.7 7 H C.sub.3 H.sub.7 0 188.5 261 27 H CHCH i-C.sub.3 H.sub.7 6 H     3-thienyl- 1 -- 262 32 H CHCH i-C.sub.3 H.sub.7 6 H 3-thienyl- 0 273.1     263 23 H CHN i-C.sub.3 H.sub.7 6 H C.sub.3 H.sub.7 0 187.3 264 23 H CHN     i-C.sub.3 H.sub.7 6 H C.sub.2 H.sub.5 0 194.8 265 27 H CHN i-C.sub.3     H.sub.7 6 H 2-thienyl- 1 -- 266 32 H CHN i-C.sub.3      H.sub.7 6 H 2-thienyl- 0 >300(dec.) 267 27 H CHCH i-C.sub.3 H.sub.7 6 H     2Cl6FC.sub.6 H.sub.3 1 -- 268 32 H CHCH i-C.sub.3      H.sub.7 6 H 2Cl6FC.sub.6 H.sub.3 0 165.4 269 27 H CHCH i-C.sub.3     H.sub.7 6 H 3Br4OHC.sub.6 H.sub.3 1 -- 270 32 H CHCH i-C.sub.3 H.sub.7 6     H 3Br4OHC.sub.6 H.sub.3 0 241.4 271 20 H CHCH i-C.sub. 3 H.sub.7 7 H     3-pyridinyl- 0 294.2 272 27 H CHN C.sub.2 H.sub.5 6 H C.sub.6 H.sub.5 1     -- 273 32 H CHN C.sub.2 H.sub.5 6 H C.sub.6 H.sub.5 0 272.0 274 27 H CHN     i-C.sub.4 H.sub.9 6 H C.sub.6 H.sub.5 1 -- 275 32 H CHN i-C.sub.4     H.sub.9 6 H C.sub.6 H.sub.5 0 220.1 276 27 H NCH i-C.sub.3 H.sub.7 6 H   C     4F.sub.6 H.sub.4 1 -- 277 32 H NCH i-C.sub.3 H.sub.7 6 H 4FC.sub.6     H.sub.4 0 250.0 278 27 H CHN C.sub.4 H.sub.9 6 H 3FC.sub.6 H.sub.4 1 --     279 32 H CHN C.sub.4 H.sub.9 6 H 3FC.sub.6 H.sub.4 0 132.9 280 23 H CHN     i-C.sub.3 H.sub.7 7 H C.sub.2 H.sub.5 0 163.9/0.5H.sub.2 O 281 27 H CHCH     i-C.sub.3 H.sub.7 6 H 3Cl4OHC.sub.6 H.sub.3 1 -- 282 32 H CHCH i-C.sub.3     H.sub.7 6 H 3Cl4OHC.sub.6 H.sub.3 0 237.0 283 20 H CHN i-C.sub.3 H.sub.7     6 H 3-pyridinyl- 0 236.3 284 27 H NCH i-C.sub.3 H.sub.7 6 H C.sub.6     H.sub.5 1 -- 285 32 H NCH i-C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 0 210.2     286 27 H CHN C.sub.3 H.sub.7 6 H 2-CH.sub.3C.sub.6 H.sub.4 1 -- 287 32 H C     HN C.sub.3 H.sub.7 6 H 2-CH.sub.3C.sub.6 H.sub.4 0 230.8 288 27 H  NCH     i-C.sub.3 H.sub.7 6 H 3ClC.sub.6 H.sub.4 1 -- 289 32 H NCH i-C.sub.3     H.sub.7 6 H 3ClC.sub.6 H.sub.4 0 176.7 290 27 H CHN i-C.sub.4 H.sub.9 6     H 2CH.sub.3C.sub.6 H.sub.4 1 -- 291 32 H CHN i-C.sub.4 H.sub.9 6 H     2CH.sub.3C.sub.6 H.sub.4 0 168.3 292 27 H CHN C.sub.2 H.sub.5 6 H     2CH.sub.3C.sub.6 H.sub.4 1 -- 293 32 H CHN C.sub.2      H.sub.5 6 H 2CH.sub.3C.sub.6 H.sub.4 0 187.0 294 27 H CHN i-C.sub.3     H.sub.7 6 H 3ClC.sub.6 H.sub.4 1 -- 295 32 H CHN i-C.sub.3 H.sub.7 6 H     3ClC.sub.6 H.sub.4 0 183.6 296 27 H  NCH i-C.sub.3 H.sub.7 6 H 3FC.sub.6     H.sub.4 1 -- 297 32 H NCH i-C.sub.3 H.sub.7 6 H 3FC.sub.6 H.sub.4 0     213.2 298 27 H CHN i-C.sub.3 H.sub.7 6 H 2FC.sub.6 H.sub.4 1 -- 299 32 H C     HN i-C.sub.3 H.sub.7 6 H 2FC.sub.6 H.sub.4 0 125.8 300 27 H CHN C.sub.4     H.sub.9 6 H C.sub.6 H.sub.5 1 -- 301 32 H CHN C.sub.4 H.sub.9 6 H     C.sub.6 H.sub.5 0 183.3 302 27 H CHN C.sub.4      H.sub.9 6 H 2-CH.sub.3C.sub.6 H.sub.4 1 -- 303 32 H CHN C.sub.4 H.sub.9     6 H 2-CH.sub.3C.sub.6 H.sub.4 0 176.4 304 27 H CHN C.sub. 3 H.sub.7 6 H     3FC.sub.6 H.sub.4 1 -- 305 32 H CHN C.sub.3 H.sub.7 6 H 3FC.sub.6     H.sub.4 1 210.5 306 27 H CHN C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 1 --     307 32 H CHN C.sub.3 H.sub.7 6 H C.sub.6 H.sub.5 0 206.3 308 27 H CHCH     C.sub.2 H.sub.5 6 H 2-CH.sub.3C.sub.6 H.sub.4 1 -- 309 32 H CHCH C.sub.2     H.sub.5 6 H 2-CH.sub.3C.sub.6 H.sub.4 0 202.2 310 -- H NCH i-C.sub.3     H.sub.7 6 H CH.sub.3 0 -- 311 -- H CHCH 1.sub.--H-imidazolyl 6 H     CH.sub.3 0 -- 312 -- H CHCH i-C.sub.3 H.sub.7 6 C.sub.6 H.sub.5CH.sub.2     CH.sub.3 0 -- 313 -- H CHCH 3ClC.sub.6 H.sub.4 7 H i-C.sub.3 H.sub.7 0     -- 314 -- H CHCH i-C.sub.3 H.sub.7 7 H C.sub.2     *= CH(CH.sub.3)COOC.sub.2 H.sub.5

                                      TABLE 10                                    __________________________________________________________________________     ##STR36##                                                                    Comp.                                                                             Ex.                         mp.(°C.)/                              No. No.                                                                              R  X.sup.1X.sup.2                                                                      Y    p R.sup.5                                                                            R.sup.6                                                                           base/salt                                     __________________________________________________________________________    315 29 H  CHCH  H    6 H    H   >300                                          316 -- H  CHCH  C.sub.6 H.sub.5                                                                    6 CH.sub.3                                                                           CH.sub.3                                                                          --                                            317 -- H  CHCH  i-C.sub.3 H.sub.7                                                                  6 CH.sub.3                                                                           CH.sub.3                                                                          --                                            __________________________________________________________________________

C. PHARMACOLOGICAL EXAMPLES

The useful pharmacological properties of the compounds of the presentinvention can for example be demonstrated by the following experiment.

EXAMPLE 39 Metabolism of exogenously administered all-trans-retinoicacid

Male Wistar rats weighing 200˜210 g were orally treated with vehicle(PEG 200) or with 40 mg/kg of a compound of formula (I). One hour later,the animals were anesthetized with ether and injected intrajugularlywith 0.50 ml saline solution containing 20 μg of all-trans-retinoicacid. Two hours after this injection, rats were killed by decapitationand blood was collected on heparin. Blood samples were centrifuged (1000g, 15 min) and plasma was recovered to determine the quantity ofplasmatic all-trans-retinoic acid. The samples were analyzed by means ofHPLC with UV-detection at 350 nm. Quantification was achieved by peakarea integration and external standardization. Under the conditionsused, plasma concentrations of the retinoic acid in vehicle-pretreatedanimals were not detectable (<0.5 ng/ml), whereas compound nos. 5, 9,11, 12, 13, 15, 16, 18, 57, 68, 69, 70, 86, 89, 94, 97, 103, 123, 132,133, 134, 141, 146, 147, 148, 149, 151, 157, 161, 181, 183, 187, 198,201, 210, 262, 263, 264, 295 and 299 enhanced the recovery ofall-trans-retinoic acid from the plasma to at least 10 ng/ml afterdosing with 40 mg/kg. The following compounds even enhanced the recoveryof all trans-retinoic acid from the plasma to at least 20 ng/ml afterdosing with 40 mg/kg: compound nos. 12, 70, 77, 86, 138 and 146.

EXAMPLE 40 Metabolism of endogenously administered all-trans-retinoicacid

Male Wistar rats weighing 200˜210 g were orally treated with vehicle(PEG 200) or with 40 mg/kg of a compound of formula (I). Two hours afterdrug administration, the rats were killed by decapitation and blood wascollected on heparin. Blood samples were centrifuged (1000 g, 15 min)and plasma was recovered to determine the quantity of plasmaticall-trans-retinoic acid. The samples were analyzed by means of HPLC withUV-detection at 350 nm. Quantification was achieved by peak areaintegration and external standardization. Under the conditions used,plasma concentrations of the retinoic acid in vehicle-pretreated animalswere not detectable (<0.5 ng/ml), whereas compound nos. 5, 77, 94, 127,151, 170, 183, 187, 190, 197, 201, 205, 208, 210, 212, 216, 218, 232,246, 259, 260, 262, 263, 264, 266, 271, 273, 275, 277, 279, 280, 285,287, 289, 291, 293, 295, 299, 301, 307 and 309 enhanced the recovery ofall-trans-retinoic acid from the plasma to at least 1 ng/ml.

We claim:
 1. A compound having the formula ##STR37## or apharmaceutically acceptable acid addition salt thereof and thestereochemically isomeric form thereof, wherein--X¹ ═X² -- is a bivalentradical having the formula

    --CH═CH--                                              (x),

    --CH═N--                                               (y),

    or

    --N═CH--                                               (z);

R is hydrogen or C₁₋₆ alkyl; Y is hydrogen; C₁₋₁₀ alkyl; C₃₋₇cycloalkyl; Ar¹ ; Ar² -C₁₋₆ alkyl; C₂₋₆ alkenyl or C₂₋₆ alkynyl; Z is aradical of formula ##STR38## wherein R¹ is hydrogen, halo, C₁₋₆ alkyl,C₁₋₆ alkyloxy, amino, mono- or di(C₁₋₆ alkyl)amino, Ar² or imidazolyl;R²is hydrogen, C₁₋₆ alkyl or Ar¹ ; R³ and R⁷ each independently arehydrogen, C₁₋₆ alkyl, Ar² -C₁₋₆ alkyl, amino or mono (C₁₋₆ alkyl)amino;R⁴ and R⁸ each independently are hydrogen, C₁₋₆ alkyl, Ar¹, C₁₋₆alkylcarbonyl, Ar² -carbonyl, C₁₋₆ alkyloxycarbonyl, carboxyl, C₁₋₆alkyloxycarbonylC₁₋₄ alkyl, aminocarbonyl or cyano; R⁵, R⁶, R⁹, R¹⁰ andR¹¹ each independently are hydrogen, C₁₋₆ alkyl or Ar² -C₁₋₆ alkyl; n is0 or 1; and Ar¹ is phenyl, substituted phenyl, naphthalenyl, pyridinyl,imidazolyl, triazolyl, thienyl, furanyl or thiazolyl and Ar² is phenylor substituted phenyl; and substituted phenyl in Ar¹ or Ar² being phenylsubstituted with 1, 2 or 3 substituents each independently selected fromhalo, hydroxy, trifluoromethyl, C₁₋₆ alkyl, C₁₋₆ alkyloxy, cyano, amino,mono- and di(C₁₋₆ alkyl)amino, nitro, carboxyl, formyl and C₁₋₆alkyloxycarbonyl.
 2. A compound according to claim 1 wherein R ishydrogen or C₁₋₄ alkyl; Y is hydrogen, C₁₋₆ alkyl, C₃₋₇ cycloalkyl,phenyl, substituted phenyl, pyridinyl, imidazolyl or thienyl; Z is aradical of formula (a-1), (a-2), (a-3), (a-4) or (a-5) wherein R¹ ishydrogen, C₁₋₄ alkyl, halo, C₁₋₄ alkyloxy, amino, mono- or di(C₁₋₄alkyl)amino, phenyl, substituted phenyl or imidazolyl; R² is hydrogen,C₁₋₄ alkyl, phenyl or substituted phenyl; R³ is hydrogen, C₁₋₄ alkyl,amino, C₁₋₄ alkylamino or C₁₋₄ alkyl substituted with phenyl orsubstituted phenyl; R⁴ is hydrogen, C₁₋₄ alkyl, C₁₋₄ alkyloxyarbonylC₁₋₄alkyl, phenyl, substituted phenyl, carboxyl, C₁₋₄ alkyloxycarbonyl,carboxyl, phenylcarbonyl, substituted phenylcarbonyl, naphthalenyl,thienyl, furanyl, pyridinyl or imidazolyl; R⁵ and R⁶ each independentlyare hydrogen or C₁₋₄ alkyl; R⁷ is hydrogen, C₁₋₄ alkyl, amino or C₁₋₄alkyl substituted with phenyl or substituted phenyl; R⁸ is hydrogen, C₁₋4 alkyl, phenyl, substituted phenyl, C₃₋₇ cycloalkyl, naphthalenyl,thienyl, pyridinyl or imidazolyl; R⁹ is hydrogen or C₁₋₄ alkyl and R¹⁰and R¹¹ are hydrogen.
 3. A compound according to claim 2 wherein --X¹═X² -- is a radical having the formula (x) or (y); R is hydrogen; and Yis hydrogen, C₁₋₄ alkyl, cyclopropyl, cyclopentyl, cyclohexyl,imidazolyl, thienyl, pyridinyl or phenyl optionally substituted with oneor two substituents selected from halo, C₁₋₄ alkyl, C₁₋₄ alkyloxy andtrifluoromethyl.
 4. A compound according to claim 3 whereinZ is aradical of formula (a-1) wherein R¹ is hydrogen, C₁₋₄ alkyl, C₁₋₄alkyloxy, halo, amino, di(C₁₋₄ alkyl)amino, phenyl or imidazolyl, R² ishydrogen, C₁₋₄ alkyl or phenyl and Y is hydrogen, C₁₋₄ alkyl, thienyl,imidazolyl or phenyl optionally substituted with one or two substituentsselected from halo, C₁₋₄ alkyl, C₁₋₄ alkyloxy or trifluoromethyl; or Zis a radical of formula (a-2) wherein R³ is hydrogen, C₁₋₄ alkyl, aminoor C₁₋₄ alkyl substituted with phenyl and R⁴ is hydrogen, C₁₋₄ alkyl,carboxyl, C₁₋₄ alkyloxycarbonyl, naphthalenyl, thienyl, pyridinyl,imidazolyl, phenyl or phenyl substituted with 1, 2 or 3 substituentseach independently selected from C₁₋₄ alkyl, C₁₋₄ alkyloxy, halo,hydroxy and trifluoromethyl and Y is hydrogen, C₁₋₄ alkyl, cyclopropyl,cyclopentyl, cyclohexyl, imidazolyl, thienyl, pyridinyl or phenyloptionally substituted with one or two substituents selected from halo,C₁₋₄ alkyl, C₁₋₄ alkyloxy and trifluoromethyl.
 5. A compound accordingto claim 1 wherein Z is a radical of formula (a-1); --X¹ ═X² -- is aradical having the formula (x) or (y); R is hydrogen; R¹ and R² are bothhydrogen and Y is phenyl, halophenyl or thienyl.
 6. A compound accordingto claim 1 wherein Z is a radical of formula (a-2); --X¹ ═X² -- is aradical of formula (x) or (y); R is hydrogen; Y is hydrogen, C₁₋₄ alkyl,cyclopropyl, cyclopentyl or cyclohexyl; R³ is hydrogen; R⁴ is hydrogen,C₁₋₄ alkyl, naphthalenyl, thienyl, pyridinyl, imidazolyl, phenyl orphenyl substituted with 1 or 2 substituents each independently selectedfrom methyl, halo, hydroxy and methoxy; and n is
 0. 7. A compoundaccording to claim 1 wherein Z is a radical of formula (a-2); --X¹ ═X²-- is a radical of formula (x) or (y); R is hydrogen; Y is phenyl orhalophenyl; R³ is hydrogen; R⁴ is hydrogen or C₁₋₄ alkyl and n is
 0. 8.A compound according to claim 1 wherein the compound is6-[(1H-imidazol-1-yl) phenylmethyl]quinoxaline and 6-[(4-fluorophenyl)(1H-imidazol-1-yl)methyl]quinoxaline, a pharmaceutically acceptable acidaddition salt or a possible stereoisomeric form thereof.
 9. A compoundaccording to claim 1 wherein the compound is6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-phenyl-2(1H)-quinoxalinone,6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-3-propyl-2(1H)-quinoxalinone,3-(3-fluorophenyl)-6-[1-(1H-imidazol-1-yl)-2-methylpropyl]-2(1H)-quinoxalinone,a pharmaceutically acceptable acid addition salt or a possiblestereoisomer thereof.
 10. A retinoid metabolism inhibiting compositioncomprising an inert carrier and as active ingredient an effective amountof a compound as defined in claim
 1. 11. A composition according toclaim 10 wherein R is hydrogen or C₁₋₄ alkyl; Y is hydrogen, C₁₋₆ alkyl,C₃₋₇ cycloalkyl, phenyl, substituted phenyl, pyridinyl, imidazolyl orthienyl; Z is a radical of formula (a-1), (a-2), (a-3), (a-4) or (a-5)wherein R¹ is hydrogen, C₁₋₄ alkyl, halo, C₁₋₄ alkyloxy, amino, mono- ordi(C₁₋₄ alkyl)amino, phenyl, substituted phenyl or imidazolyl, R² ishydrogen, C₁₋₄ alkyl, phenyl or substituted phenyl, R³ is hydrogen, C₁₋₄alkyl, amino, C₁₋₄ alkylamino or C₁₋₄ alkyl substituted with phenyl orsubstituted phenyl; R⁴ is hydrogen, C₁₋₄ alkyl, C₁₋₄ alkyloxyarbonylC₁₋₄alkyl, phenyl, substituted phenyl, carboxyl, C₁₋₄ alkyloxycarbonyl,carboxyl, phenylcarbonyl, substituted phenylcarbonyl, naphthalenyl,thienyl, furanyl, pyridinyl or imidazolyl; R⁵ and R⁶ each independentlyare hydrogen or C₁₋₄ alkyl; R⁷ is hydrogen, C₁₋₄ alkyl, amino or C₁₋₄alkyl substituted with phenyl or substituted phenyl; R⁸ is hydrogen,C₁₋₄ alkyl, phenyl, substituted phenyl, C₃₋₇ cycloalkyl, naphthalenyl,thienyl, pyridinyl or imidazolyl; R⁹ is hydrogen or C₁₋₄ alkyl and R¹⁰and R¹¹ are hydrogen.
 12. A composition according to claim 12 wherein--X¹ ═X² -- is a radical having the formula (x) or (y); R is hydrogen;and Y is hydrogen, C₁₋₄ alkyl, cyclopropyl, cyclopentyl, cyclohexyl,imidazolyl, thienyl, pyridinyl or phenyl optionally substituted with oneor two substituents selected from halo, C₁₋₄ alkyl, C₁₋₄ alkyloxy andtrifluoromethyl.
 13. A composition according to claim 12 whereinZ is aradical of formula (a-1) wherein R¹ is hydrogen, C₁₋₄ alkyl, C₁₋₄alkyloxy, halo, amino, di(C₁₋₄ alkyl)amino, phenyl or imidazolyl, R² ishydrogen, C₁₋₄ alkyl or phenyl and Y is hydrogen, C₁₋₄ alkyl, thienyl,imidazolyl or phenyl optionally substituted with one or two substituentsselected from halo, C₁₋₄ alkyl, C₁₋₄ alkyloxy or trifluoromethyl; or Zis a radical of formula (a-2) wherein R³ is hydrogen, C₁₋₄ alkyl, aminoor C₁₋₄ alkyl substituted with phenyl and R⁴ is hydrogen, C₁₋₄ alkyl,carboxyl, C₁₋₄ alkyloxycarbonyl, naphthalenyl, thienyl, pyridinyl,imidazolyl, phenyl or phenyl substituted with 1, 2 or 3 substituentseach independently selected from C₁₋₄ alkyl, C₁₋₄ alkyloxy, halo,hydroxy and trifluoromethyl and Y is hydrogen, C₁₋₄ alkyl, cyclopropyl,cyclopentyl, cyclohexyl, imidazolyl, thienyl, pyridinyl or phenyloptionally substituted with one or two substituents selected from halo,C₁₋₄ alkyl, C₁₋₄ alkyloxy and trifluoromethyl.